AW: [Histonet] (no subject)

[Gudrun Lang]

Hi Aazath,
This is our HE protocol, it's for 16 stations. 
And I'm interested in your PAP-protocol. Would you be so nice and send it to
me?

25 min oven
2 x 5 min Xylol-substitute
1 x 1 min 96%
1 x 1 min 80%
1 x 1 min 50%
1 x 1 min dest. water
1 x 10 min Mayers Hemalaun (exact)
1 x 5 min tapwater running
1 x 30 sek 2% Eosin in water (exact)
1 x 30 sek tapwater running (exact)
1 x 30 sek 96% (exact)
3 x 1 min 100%
2 x Xylol-substitute
Coverslipper

The times without "exact" are minimum, "dip" is on with all stations.
Alcohols and Xylol-subst. are changed every day.

Hope this helps
Gudrun Lang


-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Aazath Raj
Gesendet: Samstag, 29. August 2009 19:51
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] (no subject)


Dear All,
         we have got a new leica multistainer ST 5020.we are using it for
both H&E and as well as PAP.Out of 34 station i have only 15 station for
H&E.Is anybody having a Staining protocol for H&E which fits in 15 station
with 2 water wash bath.



Aazath 
Technical Officer
Apollo Hospitals Chennai
India 

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