AW: [Histonet] DIF tissue in GLUT

[Gudrun Lang]

Anne,
we do DIF on formalin fixed renal biopsies routinly. To get good results we
perform Protease digestion before DIF. I don't have the protocol here, but
we use Proteinase K from Dako at RT for 3-4 min, and the antibodies are
between 1:20 and 1:40 für 30 min.
I don't know, if that would help with glutaraldehyde-fixed specimen, but
worth trying.

Gudrun Lang
Akh Linz, Austria


-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Anne van
Binsbergen
Gesendet: Donnerstag, 27. August 2009 12:24
An: John Kiernan; histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] DIF tissue in GLUT

Dear John and other knowledgeable Histonetters and Gurus of the trade....

Renal bx tissue core has been placed in Gluteraldehyde/Trumps EM
fixative by mistake (we all make mistakes) - we now need to do DIF
Does anyone out there have a method for this?

-- 
Anne van Binsbergen (Hope)
Abu Dhabi
UAE
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