[Histonet] RE: Histonet Digest, Vol 69, Issue 7
maureen bukhari
mlbukhar <@t> ucalgary.ca
Wed Aug 26 11:59:56 CDT 2009
I just purchased some from the VWR Catalogue, page 538, top left hand
corner, disposable and reusable, 2009-2010 catalogue and you can access it
online.
Cheers,
Maureen Bukhari
Phone: 403-210-6524
e-mail: mlbukhar <@t> ucalgary.ca
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Friday, August 07, 2009 11:01 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 69, Issue 7
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Today's Topics:
1. Core unit support (Va Paula Sicurello)
2. metal embedding base molds (Victoria Baker)
3. Support for core units (Va Paula Sicurello)
4. Re: metal embedding base molds (Amy Porter)
5. Mycoplasma bovis (Breeden, Sara)
6. RE: metal embedding base molds (Bernice Frederick)
7. Re: Mycoplasma bovis (Jenee.S.Odani <@t> hawaii.gov)
8. (no subject) (Maria Katleba)
9. RE: metal embedding base molds (Nancy Klemme)
10. RE: metal embedding base molds (Jodie Robertson)
11. cement in bone specimen (DiCarlo, Margaret)
12. Re: Histonet Digest, Vol 69, Issue 6
(Rick.Garnhart <@t> memorialhealthsystem.com)
13. What do you use for decolorizing after Hematox.
(Michelle MacVeigh-Aloni)
14. RE: cement in bone specimen (Jack Ratliff)
15. Fetal demise placenta (mtitford <@t> aol.com)
16. HPGD, PAFa and frizzled-4 stainings on Ventana Benchmark
(Vesterinen Tiina)
----------------------------------------------------------------------
Message: 1
Date: Thu, 6 Aug 2009 10:12:31 -0700 (PDT)
From: Va Paula Sicurello <vapatpxs <@t> yahoo.com>
Subject: [Histonet] Core unit support
To: MSA BB <Microscopy <@t> Microscopy.Com>, HistoNet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <554043.34139.qm <@t> web46112.mail.sp1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello Everybody,
I was wondering what type of institutional support y'all have at your
various institutions. I work for a research foundation and they want to
drop my salary and have the researchers grants pay my salary and almost
everything else. I am a fee-for-service lab and the CEO wants to see $25K
in recharges between now and December.
We offer TEM, confocal, histology and cryosectioning. We will be adding
micro-CT and a deconvolution microscope that does FRET and can handle
samples that have been transfected(?) with viral vectors.
Is she being unrealistic? I am only 80% time and the only person down here.
Should I start looking for a new position before she shuts me down? How
many people have had their facilities closed by the people who don't see the
big picture?
Please let me know your experiences.
Thanks,
Paula :-}
Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core for Micro Imaging(C-MI)
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397
C-MI for your imaging needs.
------------------------------
Message: 2
Date: Thu, 6 Aug 2009 13:13:06 -0400
From: Victoria Baker <bakevictoria <@t> gmail.com>
Subject: [Histonet] metal embedding base molds
To: histonet <histonet <@t> pathology.swmed.edu>
Message-ID:
<4f016b690908061013r6a410546j8a867e9813392a69 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi-
I'm trying to locate embedding molds that Sakura/Tissue Tek makes. All I'm
able to find at this point are the metal molds with the 'wings' on them.
Does anyone know a source? With all the mergers etc it's been a little
challenging.
Thanks in advance
Vikki
------------------------------
Message: 3
Date: Thu, 6 Aug 2009 10:14:19 -0700 (PDT)
From: Va Paula Sicurello <vapatpxs <@t> yahoo.com>
Subject: [Histonet] Support for core units
To: MSA BB <Microscopy <@t> Microscopy.Com>, HistoNet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <73199.21406.qm <@t> web46104.mail.sp1.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Hello Everybody,
I was wondering what type of institutional support y'all have at your
various institutions. I work for a research foundation and they want to
drop my salary and have the researchers grants pay my salary and almost
everything else. I am a fee-for-service lab and the CEO wants to see $25K
in recharges between now and December.
We offer TEM, confocal, histology and cryosectioning. We will be adding
micro-CT and a deconvolution microscope that does FRET and can handle
samples that have been transfected(?) with viral vectors.
Is she being unrealistic? I am only 80% time and the only person down here.
Should I start looking for a new position before she shuts me down? How
many people have had their facilities closed by the people who don't see the
big picture?
Please let me know your experiences.
Thanks,
Paula :-}
Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core for Micro Imaging(C-MI)
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397
C-MI for your imaging needs.
------------------------------
Message: 4
Date: Thu, 6 Aug 2009 13:30:34 -0400
From: "Amy Porter" <portera <@t> msu.edu>
Subject: Re: [Histonet] metal embedding base molds
To: "Victoria Baker" <bakevictoria <@t> gmail.com>, "histonet"
<histonet <@t> pathology.swmed.edu>
Message-ID: <08DEAF2AEF394D02817D86F01AD998AE <@t> histolab>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
Try EM Sciences the distribute alot of the unique old type histology
supplies.
Amy S. Porter, HT (ASCP) QIHC
Investigative HistoPathology Laboratory - Supervisor
2201 Biomedical Physical Sciences Bldg. Rm #2133
East Lansing, MI 48824-3320
Phone: (517) 884-5026
Fax: (517) 432-1368
Email: portera <@t> msu.edu
Web: www.humanpathology.msu.edu
----- Original Message -----
From: "Victoria Baker" <bakevictoria <@t> gmail.com>
To: "histonet" <histonet <@t> pathology.swmed.edu>
Sent: Thursday, August 06, 2009 1:13 PM
Subject: [Histonet] metal embedding base molds
> Hi-
>
> I'm trying to locate embedding molds that Sakura/Tissue Tek makes. All
> I'm
> able to find at this point are the metal molds with the 'wings' on them.
>
> Does anyone know a source? With all the mergers etc it's been a little
> challenging.
>
> Thanks in advance
>
> Vikki
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 5
Date: Thu, 6 Aug 2009 12:08:44 -0600
From: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Subject: [Histonet] Mycoplasma bovis
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4D14F0FC9316DD41972D5F03C070908B02E46A05 <@t> nmdamailsvr.nmda.ad.nmsu.edu>
Content-Type: text/plain; charset="US-ASCII"
My boss has thought up a new project for me so I need to know what I
need to know about Mycoplasma bovis for IHC. He wants me to cost out
the procedure. I normally do only TWO IHCs - CWD and BVD - so I'll be
charting new mental territory with this one. Can anyone guide me on
where to start figuring this out? Where do I get antibody? Is there a
pre-existing protocol for the Ventana NexES? I don't believe in
re-inventing the wheel if I don't need to. Thanks!
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM 87106
505-841-2576
------------------------------
Message: 6
Date: Thu, 6 Aug 2009 13:10:38 -0500
From: "Bernice Frederick" <b-frederick <@t> northwestern.edu>
Subject: RE: [Histonet] metal embedding base molds
To: "'Amy Porter'" <portera <@t> msu.edu>, "'Victoria Baker'"
<bakevictoria <@t> gmail.com>, "'histonet'"
<histonet <@t> pathology.swmed.edu>
Message-ID: <B57F6F56230440F0AA69125A641AB29F <@t> lurie.northwestern.edu>
Content-Type: text/plain; charset="us-ascii"
As well as surgipath.
Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amy Porter
Sent: Thursday, August 06, 2009 12:31 PM
To: Victoria Baker; histonet
Subject: Re: [Histonet] metal embedding base molds
Try EM Sciences the distribute alot of the unique old type histology
supplies.
Amy S. Porter, HT (ASCP) QIHC
Investigative HistoPathology Laboratory - Supervisor
2201 Biomedical Physical Sciences Bldg. Rm #2133
East Lansing, MI 48824-3320
Phone: (517) 884-5026
Fax: (517) 432-1368
Email: portera <@t> msu.edu
Web: www.humanpathology.msu.edu
----- Original Message -----
From: "Victoria Baker" <bakevictoria <@t> gmail.com>
To: "histonet" <histonet <@t> pathology.swmed.edu>
Sent: Thursday, August 06, 2009 1:13 PM
Subject: [Histonet] metal embedding base molds
> Hi-
>
> I'm trying to locate embedding molds that Sakura/Tissue Tek makes. All
> I'm
> able to find at this point are the metal molds with the 'wings' on them.
>
> Does anyone know a source? With all the mergers etc it's been a little
> challenging.
>
> Thanks in advance
>
> Vikki
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Thu, 6 Aug 2009 08:19:25 -1000
From: Jenee.S.Odani <@t> hawaii.gov
Subject: Re: [Histonet] Mycoplasma bovis
To: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Cc: histonet <@t> lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OFF539D4DB.F9C49528-ON0A25760A.00648E7D-0A25760A.00643411 <@t> hawaii.gov>
Content-Type: text/plain; charset="US-ASCII"
Hi there,
Check out this database. It contains a contact list for the labs that are
currently doing the Myco bovis IHC.
http://ihc.sdstate.org/
-J
Jenee S. Odani, D.V.M., Dipl. ACVP
Veterinary Medical Officer
Hawaii State Veterinary Laboratory/DAI
99-941 Halawa Valley Street, Aiea, HI, 96701
Phone: (808) 483-7131/Fax: (808) 483-7110
"Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
08/06/2009 08:12 AM
To
<histonet <@t> lists.utsouthwestern.edu>
cc
Subject
[Histonet] Mycoplasma bovis
My boss has thought up a new project for me so I need to know what I
need to know about Mycoplasma bovis for IHC. He wants me to cost out
the procedure. I normally do only TWO IHCs - CWD and BVD - so I'll be
charting new mental territory with this one. Can anyone guide me on
where to start figuring this out? Where do I get antibody? Is there a
pre-existing protocol for the Ventana NexES? I don't believe in
re-inventing the wheel if I don't need to. Thanks!
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM 87106
505-841-2576
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Thu, 6 Aug 2009 11:23:21 -0700
From: Maria Katleba <Maria.Katleba <@t> stjoe.org>
Subject: [Histonet] (no subject)
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<97C02552ECB11346877D3E83CF833ABD13D17C4880 <@t> SJSNT-SCMAIL03.stjoe.org>
Content-Type: text/plain; charset="us-ascii"
Hi All,
Would anyone tell me what their lab procedure is for handling fetal demise
placentas?
In previous jobs sites, the histotech and/or PA would read the requisition
and determine if there may be later DNA testing ordered.
If yes, put in fridge and contact pathologist and/or physician.
If not, place in formalin.
Currently, our PA automatically places every placenta in formalin....
Kind regards,
Maria Katleba HT(ASCP), MS
Pathology Dept. Mgr.
Queen of the Valley Medical Center
707-294-9229 cell
707-252-4411 x3689
________________________________
Notice from St. Joseph Health System:
Please note that the information contained in this message may be privileged
and confidential and protected from disclosure.
------------------------------
Message: 9
Date: Thu, 6 Aug 2009 11:23:18 -0700
From: Nancy Klemme <NKlemme <@t> sakuraus.com>
Subject: RE: [Histonet] metal embedding base molds
To: Victoria Baker <bakevictoria <@t> gmail.com>, histonet
<histonet <@t> pathology.swmed.edu>
Message-ID:
<782E3A02C2EB2347BEA6DEA69DC7AB865B4BFF12E7 <@t> sfamail.SAKURAUS.LOCAL>
Content-Type: text/plain; charset="us-ascii"
Dear Vikki,
Sakura still carries the metal base molds that are used with
Embedding Rings. They do not have the "wings" that the ones for use with the
Unicassettes have. Their Sakura part numbers begin from 4121 and higher.
If you use the electronic catalog on Sakura's website (www.sakuraus.com) and
search by product description, just type in base molds. Then click on part
# 4121 and the wingless ones will be pictured.
Kind regards,
Nancy Klemme, Sakura Educational Services
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Victoria
Baker
Sent: Thursday, August 06, 2009 10:13 AM
To: histonet
Subject: [Histonet] metal embedding base molds
Hi-
I'm trying to locate embedding molds that Sakura/Tissue Tek makes. All I'm
able to find at this point are the metal molds with the 'wings' on them.
Does anyone know a source? With all the mergers etc it's been a little
challenging.
Thanks in advance
Vikki
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Thu, 6 Aug 2009 12:24:53 -0700
From: "Jodie Robertson" <jrobertson <@t> pathologysciences.com>
Subject: RE: [Histonet] metal embedding base molds
To: "Victoria Baker" <bakevictoria <@t> gmail.com>, "histonet"
<histonet <@t> pathology.swmed.edu>
Message-ID:
<518CD6920AA7154193CBE5977CD880733A8AFE <@t> psmgsrv2.PSMG.local>
Content-Type: text/plain; charset="us-ascii"
Try Cardinal as they are a distributor for all Tissue Tek/Sakura
supplies.
Jodie Robertson, HT(ASCP)QIHC
Histology Day Supervisor
Pathology Sciences Medical Group
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Victoria
Baker
Sent: Thursday, August 06, 2009 10:13 AM
To: histonet
Subject: [Histonet] metal embedding base molds
Hi-
I'm trying to locate embedding molds that Sakura/Tissue Tek makes. All
I'm
able to find at this point are the metal molds with the 'wings' on them.
Does anyone know a source? With all the mergers etc it's been a little
challenging.
Thanks in advance
Vikki
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Thu, 6 Aug 2009 15:43:16 -0400
From: "DiCarlo, Margaret" <MDiCarlo <@t> KaleidaHealth.Org>
Subject: [Histonet] cement in bone specimen
To: <histonet <@t> pathology.swmed.edu>
Message-ID:
<1B73766A27A1554CB2729B6432E81301015C76BA <@t> KALEXMB04.KaleidaHealth.org>
Content-Type: text/plain; charset="US-ASCII"
Histonetters,
We sliced a humerus bone on a band saw which was filled with cement from
a previous surgery and my boss would like me to try to cut it on a
sliding/sledge microtome. (The knife remains stationary) Is this
possible to do? Presently, it is fixing and then I will decal it but
I'm wondering if I'll be wasting my time and effort.
I would appreciate anyone's experience with this.
Thank you.
Peggy DiCarlo HT (ASCP)
Orthopedic Bone Lab
Buffalo General Hospital
Buffalo, NY 14203
716-859-1293
2009 Best Places to Work Winner
Visit our careers page at www.kaleidahealth.org/careers
CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or
previous e-mail messages attached to it are confidential and intended solely
for the use of the individual or entity to whom they are addressed. If you
are not the intended recipient, or a person responsible for delivering it to
the intended recipient, you are hereby notified that any further review,
disclosure, copying, dissemination, distribution, or use of any of the
information contained in or attached to this e-mail transmission is strictly
prohibited. If you have received this message in error, please notify the
sender immediately by e-mail, discard any paper copies, and delete all
electronic files of the message. If you are unable to contact the sender or
you are not sure as to whether you are the intended recipient, please e-mail
ISTSEC <@t> KaleidaHealth.org or call (716) 859-7777.
------------------------------
Message: 12
Date: Thu, 6 Aug 2009 14:03:11 -0600
From: Rick.Garnhart <@t> memorialhealthsystem.com
Subject: [Histonet] Re: Histonet Digest, Vol 69, Issue 6
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OFF5871773.D62F0E69-ON8725760A.006DDFA9-8725760A.006E2682 <@t> memorialhealthsys
tem.com>
Content-Type: text/plain; charset=US-ASCII
Does anyone out in histology have any documentation from CMS, ASCP, or NSH
that breaks down the job duties or areas for what is covered under Tech
and professional part of the CPT billing codes. For example is Grossing part
of the a profee or tech part of the bill.
Rick Garnhart HT(ASCP)
Memorial Health System
Histology Supervisor
1400 E. Boulder St.
Colorado Springs, CO 80909
Cell: 719-365-8357
Ph: 719-365-6926
Fax: 719-365-6373
rick.garnhart <@t> memorialhealthsystem.com
Mission: To provide the highest quality health care
Vision: To create an outstanding health system where patients heal and
people thrive
Values: Compassion - Integrity - Quality - Respect - Teamwork
www.memorialhealthsystem.com
The information contained in or attached to this electronic message is
privileged and confidential, intended only for the use of the individual(s)
named above. If the reader of this message is not the intended recipient, or
the employee or agent responsible to deliver it to the intended
recipient, you are hereby notified that any dissemination, distribution, or
copying of this communication is strictly prohibited. If you have received
this communication in error, please inform the sender immediately and remove
any record of this message.
------------------------------
Message: 13
Date: Thu, 6 Aug 2009 13:03:33 -0700
From: "Michelle MacVeigh-Aloni" <macveigh <@t> usc.edu>
Subject: [Histonet] What do you use for decolorizing after Hematox.
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <002401ca16d0$f9e4a3c0$5c237d80 <@t> DFS66DD1>
Content-Type: text/plain; charset="iso-8859-1"
Hi all,
In my H&E staining I always used 1% acid alcohol. (Working with conc. HCl is
not fun!)
There is the Orth's solution available already made, but it is 3%. Do you
use that? How do you use it?
What do you use in your regulat H&E stain? I am using it on an old Jung
AUTOSTAINER.
Michelle
Research Specialist
USC Keck School of Medicine
------------------------------
Message: 14
Date: Thu, 6 Aug 2009 16:21:41 -0400
From: Jack Ratliff <ratliffjack <@t> hotmail.com>
Subject: RE: [Histonet] cement in bone specimen
To: <mdicarlo <@t> kaleidahealth.org>, <histonet <@t> pathology.swmed.edu>
Message-ID: <BLU108-W219BE87505BA8C878B7F82AE0A0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Peggy,
If the cement you mention is or similar to PMMA, then you will not be able
to cut thin sections using the sliding/sledge microtome. If I was you, I
would not decal it at all as it will only make the bone less dense than the
cement and will definitely waste your time if you embed it in paraffin to
try and cut later. Your best and only chance at success here is to embed
undemineralized in resin and cut thick or ground sections that can be
polished to a desired thickness.
If you would like to discuss this further, please give me a call at
317-281-1975.
Best,
Jack Ratliff
> Date: Thu, 6 Aug 2009 15:43:16 -0400
> From: MDiCarlo <@t> KaleidaHealth.Org
> To: histonet <@t> pathology.swmed.edu
> CC:
> Subject: [Histonet] cement in bone specimen
>
> Histonetters,
>
>
>
> We sliced a humerus bone on a band saw which was filled with cement from
> a previous surgery and my boss would like me to try to cut it on a
> sliding/sledge microtome. (The knife remains stationary) Is this
> possible to do? Presently, it is fixing and then I will decal it but
> I'm wondering if I'll be wasting my time and effort.
>
>
>
> I would appreciate anyone's experience with this.
>
>
>
> Thank you.
>
>
>
> Peggy DiCarlo HT (ASCP)
>
> Orthopedic Bone Lab
>
> Buffalo General Hospital
>
> Buffalo, NY 14203
>
> 716-859-1293
>
>
>
>
>
> 2009 Best Places to Work Winner
> Visit our careers page at www.kaleidahealth.org/careers
>
> CONFIDENTIALITY NOTICE: This email transmission and any documents, files,
or previous e-mail messages attached to it are confidential and intended
solely for the use of the individual or entity to whom they are addressed.
If you are not the intended recipient, or a person responsible for
delivering it to the intended recipient, you are hereby notified that any
further review, disclosure, copying, dissemination, distribution, or use of
any of the information contained in or attached to this e-mail transmission
is strictly prohibited. If you have received this message in error, please
notify the sender immediately by e-mail, discard any paper copies, and
delete all electronic files of the message. If you are unable to contact the
sender or you are not sure as to whether you are the intended recipient,
please e-mail ISTSEC <@t> KaleidaHealth.org or call (716) 859-7777.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 15
Date: Thu, 06 Aug 2009 16:53:16 -0400
From: mtitford <@t> aol.com
Subject: [Histonet] Fetal demise placenta
To: Histonet <@t> pathology.swmed.edu
Message-ID: <8CBE4EF0E4C2479-93C-E73 <@t> webmail-me19.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
Maria asks about fetal demise placenta:
We gross in all fetal demise placenta (usually four blocks).If the
clinicians desire genetics, they take?samples from the fetus and the
placenta up on the floor. (There is only a three day window for growing
fibroblasts).
The fetus gets a gross only treatment with weight, measurements and a
photograph. If the clinicians or family desire an autopsy, whatever the
gestational age, we have the parents sign an autopsy consent, as if for an
adult.
Regards
Michael Titford
USA Pathology
Mobile AL
------------------------------
Message: 16
Date: Fri, 7 Aug 2009 09:05:35 +0300
From: Vesterinen Tiina <Tiina.Vesterinen <@t> hus.fi>
Subject: [Histonet] HPGD, PAFa and frizzled-4 stainings on Ventana
Benchmark
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<2C0982D85C07A34598393F0B2B6F9675413886824E <@t> vanhusmbx06.hus.fi>
Content-Type: text/plain; charset="iso-8859-1"
Hi everyone,
Has anyone stained formalin fixed, paraffin embedded slices with HPGD
(Sigma), PAF acetylhydrolase (Cayman Chemical) or frizzled-4 (Santa Cruz) on
Ventana Discovery, Benchmark or similar autostainer? I would appreciate all
advices related to staining protocols because I4ve quite small amounts of
antibodies.
Regards, Tiina Vesterinen
Tiina Vesterinen
Project Researcher
Institute for Molecular Medicine Finland FIMM
Helsinki, Finland
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End of Histonet Digest, Vol 69, Issue 7
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