[Histonet] reduction/elimination of red cell autofluorescence on FFPE sections for IF examination

[Thurby, Christina]

Can anyone give feedback on reagents/procedures to use for immunofluorescence to reduce/eliminate red cell autofluorescence on FFPE sections.  I am using an indirect method of labeling for two antibodies (FITC and Texas Red).

I have not tried 0.1% sodium borohydride.  Will this help for formalin fixed specimens?  I have read that it is used for gluteraldehyde autofluorescence reduction.  If appropriate how long should this reagent be applied to the specimen and at what temperature.

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