[Histonet] FW: clearing agent for gross specimens

[Della Speranza, Vinnie]

Vinnie Della Speranza
Manager for Anatomic Pathology Services
165 Ashley Avenue Suite 309
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974
 

-----Original Message-----
From: Schneider, Dawn [mailto:Dawn.Schneider <@t> ministryhealth.org] 
Sent: Friday, August 21, 2009 3:37 PM
To: Della Speranza, Vinnie
Subject: RE: clearing agent for gross specimens

We make our own. Have been using the same one since I before I came here 17 years ago.
Here is the recipe:

Chloroform (we buy in gallon jug from Fisher)  600 ml.
Reagent Alcohol                                      300 ml
Glacial Acetic Acid                                  100 ml.
We usually make up a double batch so we have enough on hand.  I have seen the lymph nodes pop out in 30 minutes and have one pathologist who has left the specimen in clearing overnight.  It can be used before formalin fixation or after.
They use mostly for colon fat that they have trimmed off the colon.  We rinse with water for 5 minutes before grossing.

Good luck Vinnie.

Dawn D. Schneider, HT(ASCP)
Howard Young Medical Center
Woodruff, WI  54568
(715)356-8174
dawn.schneider <@t> ministryhealth.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie
Sent: Friday, August 21, 2009 12:22 PM
To: Histonet
Subject: [Histonet] FW: clearing agent for gross specimens



Vinnie Della Speranza
Manager for Anatomic Pathology Services
165 Ashley Avenue Suite 309
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974


-----Original Message-----
From: Cazares, Ruth [mailto:RCazares <@t> schosp.org]
Sent: Friday, August 21, 2009 12:58 PM
To: Della Speranza, Vinnie
Subject: RE: clearing agent for gross specimens

Vinnie,

We make our own Davidson's fixative, and we also tried Dissect Aid. Comparing the two, our PA and our pathologists liked the homemade Davidsons fixative better than the Dissect Aid.


Ruth Cazares, HT (ASCP)
Histology Supervisor
Department of Pathology
Swedish Covenant Hospital
5145 North California Ave
Chicago, IL 60625

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie
Sent: Friday, August 21, 2009 11:16 AM
To: Histonet
Subject: [Histonet] clearing agent for gross specimens

I would appreciate hearing from anyone who routinely utilizes a clearing agent in the gross room to clear fat from tissues to allow lymph nodes to be visualized.

I am aware of Dissect Aid but would like to learn of other comparable products.
If individuals are using home made solutions are will to share their formula I would appreciate receiving that information as well.

Our primary interest is speed of clearing for turnaround time reasons.

I am in the midst of a Histosearch search but it is slow going because most discussions refer to tissue processing or staining so it's like looking for a needle in a haystack


Thank you all

Vinnie Della Speranza
Manager for Anatomic Pathology Services
165 Ashley Avenue Suite 309
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974



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