[Histonet] Evan's Blue - blood brain barrier

[John Kiernan]

Because most of it it dissolves.
 
John Kiernan 
Anatomy, UWO
London, Canada
= = =
----- Original Message -----
From: "Connolly, Brett M" <brett_connolly <@t> merck.com>
Date: Friday, August 21, 2009 9:27
Subject: RE: [Histonet] Evan's Blue - blood brain barrier
To: John Kiernan <jkiernan <@t> uwo.ca>


> Thanks for the history lesson...so why is EB not well suited for examination on sections??
>  
> Brett M. Connolly, Ph.D. 
> Research Fellow, Imaging Dept. 
> Merck & Co., Inc. 
> PO Box 4, WP-44K 
> West Point, PA 19486 
> tel. 215-652-2501 fax. 215-993-6803 
> brett_connolly <@t> merck.com 
> 



> From: John Kiernan [mailto:jkiernan <@t> uwo.ca] 
> Sent: Thursday, August 20, 2009 4:24 PM
> To: Connolly, Brett M
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Evan's Blue - blood brain barrier
> 

> Evans blue (EB) and the closely similar dye trypan blue (TB) bind non-covalently to proteins, notably albumin. The dye-protein complexes are fluorescent. These dyes are OK for gross demonstration of regions with permeable capillaries (neurohypophysis, area postrema etc) They are not well suited to examination of sections, although EB was one of the first tracers of retrograde axonal transport (Kristensson et al 1971 Brain Res. 32:399-406; Kuypers et al 1977 Neurosci. Lett. 6:127-135). In early studies with trypan blue the dye was administered chronically so that some of it ended up inside cells. Quite detailed technical details were given by DF Cappell 1929 (J. Path. Bact. 32:595-708). Clasen et al 1970 (J. Neuropath. Exp. Neurol. 29:266-284) took advantage of EB-albumin fluorescence in a histological study of blood-brain barrier failure, but there are better tracers, depending on the purpose of the investigation.
>  
> John Kiernan
> Anatomy, UWO
> London, Canada
> = = =
> ----- Original Message -----
> From: "Connolly, Brett M" <brett_connolly <@t> merck.com>
> Date: Wednesday, August 19, 2009 12:19
> Subject: [Histonet] Evan's Blue - blood brain barrier
> To: histonet <@t> lists.utsouthwestern.edu
> 
> > We will be infusing Evan's Blue to assess blood brain barrier 
> > disruptionin some rats brains. The current plan is to follow 
> > this with saline
> > perfusion to clear the vasculature and then perfuse with fixative.
> > Brains will then be sunk in sucrose and frozen for sectioning, 
> > but I am
> > wondering if anyone knows if Evan's Blue withstands processing to
> > paraffin? 
> > 
> > Thanks for any tips/ info, etc.
> > 
> > Brett
> > 
> > 
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