[Histonet] bone sections

Thu Aug 20 07:58:47 CDT 2009

Jackie,

Personally, I have not used this method of adhesion for special stains
and IHC staining because I only stain for H&E. However, I know the
Pathology department here has used it in the past for both special
stains and IHC and has not had any interference or background staining.

Peggy DiCarlo HT (ASCP)
Orthopedic Bone Lab
Buffalo General Hospital
Buffalo, NY  14203
716-859-1293

-----Original Message-----
From: Garfield, Jacqueline [mailto:JGarfield <@t> lifecell.com] 
Sent: Thursday, August 20, 2009 08:17
To: DiCarlo, Margaret
Cc: Williams, Sean; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] bone sections

Peggy,

Have you used this method of adhesion for special stains and IHC
staining as well?  If so, have you had any interference or background
staining?

Thank you,
Jackie

Jacqueline D. Garfield | Manager, Histology

Main     908.947.1100                 Fax       908.947.1085
Direct   908.947.1182                 Email    jgarfield@ lifecell.com

www.lifcell.com            

LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of DiCarlo,
Margaret
Sent: Thursday, August 20, 2009 8:03 AM
To: Williams, Sean; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] bone sections

Marie,

I make a 5% Titebond solution from Titebond II which can be purchased at
any hardware store such as Home Depot, etc. and I rarely lose sections
when H&E staining on paraffin embedded bones.  

Right before sectioning, I take a pipette using the 5% titebond and dab
all the corners and center of the slide, take a kimwipe and spread it
zigzag across one way all the way down the slide and then turn the slide
1/4 and do it again.  Let it air dry a few seconds and then pick up your
section.  

I should mention that I first clean the slides in acid alcohol for 5
minutes, followed by two 95% rinses for 5 minutes each and then dry in a
60 degree oven for 1 hour.  Remove from oven, cool, wrap individually
with either a kimwipe or paper towel to keep them protected from dust
and store in an empty coverslip box.

Hope this helps.

Peggy DiCarlo HT (ASCP)
Orthopedic Bone Lab
Buffalo General Hospital
Buffalo, NY  14203
716-859-1293

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Williams, Sean
Sent: Tuesday, August 18, 2009 07:51
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] bone sections

Hi Everyone

Could any one tell me how they keep bone sections adhered to slides when
staining. I'Ve tried polysine slides, pva glue slides, different ways of
drying the sections and for different lenghts of time but nothing seems
to work for every section.
I'm only staining them with H&E.

Thanks
Marie O'Brien (liverpool vet path - histology)
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