[Histonet] bone sections

DiCarlo, Margaret MDiCarlo <@t> KaleidaHealth.Org
Thu Aug 20 07:02:34 CDT 2009


I make a 5% Titebond solution from Titebond II which can be purchased at
any hardware store such as Home Depot, etc. and I rarely lose sections
when H&E staining on paraffin embedded bones.  

Right before sectioning, I take a pipette using the 5% titebond and dab
all the corners and center of the slide, take a kimwipe and spread it
zigzag across one way all the way down the slide and then turn the slide
1/4 and do it again.  Let it air dry a few seconds and then pick up your

I should mention that I first clean the slides in acid alcohol for 5
minutes, followed by two 95% rinses for 5 minutes each and then dry in a
60 degree oven for 1 hour.  Remove from oven, cool, wrap individually
with either a kimwipe or paper towel to keep them protected from dust
and store in an empty coverslip box.

Hope this helps.

Peggy DiCarlo HT (ASCP)
Orthopedic Bone Lab
Buffalo General Hospital
Buffalo, NY  14203

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Williams, Sean
Sent: Tuesday, August 18, 2009 07:51
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] bone sections

Hi Everyone

Could any one tell me how they keep bone sections adhered to slides when
staining. I'Ve tried polysine slides, pva glue slides, different ways of
drying the sections and for different lenghts of time but nothing seems
to work for every section.
I'm only staining them with H&E.

Marie O'Brien (liverpool vet path - histology)
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

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