[Histonet] anti-GFP antibody

Adam . anonwums1 <@t> gmail.com
Wed Aug 19 19:02:23 CDT 2009


Hi all,

I am looking for a good anti-GFP antibody. I plan on using it for dual
immunofluorescence on mouse bone in paraformaldehyde fixed, paraffin
embedded sections, most likely with some antigen retrieval.

Here's the main problem: I want to use it with a goat-anti-mouse primary and
in a separate assay with a rabbit-anti-mouse antibody, both of which are
staining mouse bone. In order to save money and optimization time, I would
prefer if it were raised in a species other than goat and rabbit so I could
use it for both assays. I also want to avoid mouse antibodies to avoid the
mouse-on-mouse issue. Alternatively, I could buy two antibodies (rabbit and
goat) and use them in the respective assays. Or is there another way that's
non-obvious?

Thanks,
Adam


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