[Histonet] in situ with paraffin sections

[Emily Sours]

Hello

I've been reading a few protocols for RNA in situ hybridization with
paraffin sections.  One of the suggestions is to dehydrate the sections on
the first day after rehydration and pre-hybridization processing (para fix,
proteinase K, acetylation, pbs washes between all).  This seems counter
intuitive to me, or at least unnecessary.  Any suggestions why this would be
done?
Our protocol does not involve this, by the way, and it works fine except for
the tissue tearing.


Emily

"One of the defining characteristics of modern surgery was that patients
ought to survive it."
--Peter Stanley, For Fear of Pain: British Surgery, 1790-1850