[Histonet] Evan's Blue - blood brain barrier

Connolly, Brett M brett_connolly <@t> merck.com
Wed Aug 19 11:18:13 CDT 2009


We will be infusing Evan's Blue to assess blood brain barrier disruption
in some rats brains. The current plan is to follow this with saline
perfusion to clear the vasculature and then perfuse with fixative.
Brains will then be sunk in sucrose and frozen for sectioning, but I am
wondering if anyone knows if Evan's Blue withstands processing to
paraffin? 

Thanks for any tips/ info, etc.

Brett


Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates (which may be known
outside the United States as Merck Frosst, Merck Sharp & Dohme or
MSD and in Japan, as Banyu - direct contact information for affiliates is
available at http://www.merck.com/contact/contacts.html) that may be
confidential, proprietary copyrighted and/or legally privileged. It is
intended solely for the use of the individual or entity named on this
message. If you are not the intended recipient, and have received this
message in error, please notify us immediately by reply e-mail and
then delete it from your system.


More information about the Histonet mailing list