[Histonet] Evan's Blue - blood brain barrier

Connolly, Brett M brett_connolly <@t> merck.com
Wed Aug 19 11:18:13 CDT 2009

We will be infusing Evan's Blue to assess blood brain barrier disruption
in some rats brains. The current plan is to follow this with saline
perfusion to clear the vasculature and then perfuse with fixative.
Brains will then be sunk in sucrose and frozen for sectioning, but I am
wondering if anyone knows if Evan's Blue withstands processing to

Thanks for any tips/ info, etc.


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