[Histonet] Re: Shandon Excelsior Tissue Processor

Mon Aug 17 16:41:07 CDT 2009

Tasha,
We have the Excelsior which we received a few weeks ago.  We have not used it yet for patient specimens but will very soon.  Our test runs went well except some breast tissue (probably cut too thick).  Overall, the pathologist was happy with the results.  There are many good features, but one of them was the potential savings of alcohol solutions.  It has a built in hydrometer that measures the percentage of alcohol in the containers and it will let you know when to change it.  We are also looking into the Tissue Tek VIP.  We have the 300 model which we like but it is nearing it's life expectancy.

Garret Miyamoto
Tripler Army Medical Center

----- Original Message -----
From: histonet-request <@t> lists.utsouthwestern.edu
Date: Saturday, August 15, 2009 7:03 am
Subject: Histonet Digest, Vol 69, Issue 15
To: histonet <@t> lists.utsouthwestern.edu

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> Today's Topics:
> 
>   1. streptavidin peroxidase (MKing)
>   2. HT opening in Colorado (Matthew Lunetta)
>   3. unsubscribe (Woodward, Denise)
>   4. Job Opening  (Konni Black)
>   5. Shandon Excelsior Tissue Processor (Tasha Fraser)
>   6. Can DAB block an epitope?? (Amos Brooks)
> 
> 
> -------------------------------------------------------------------
> ---
> 
> Message: 1
> Date: Fri, 14 Aug 2009 13:16:40 -0400
> From: MKing <
> Subject: [Histonet] streptavidin peroxidase
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
> 
> Sigma E-2886 extravidin peroxidase, 1:1000 in 0.1 M PBS or sodium 
> acetate on almost every IHC variation (fixation, species, primary) we 
> do.  Supposed to be less nonspecific binding than streptavidin.
> 
> Mike King
> UF Pharmacology & Therapeutics
> 
> ------------------------------
> 
> Message: 27
> Date: Fri, 14 Aug 2009 09:56:56 -0700
> From: "Karen Cai" <
> Subject: [Histonet] Need help on Streptavidin-HRP conjugate for IHC
> To: <
> Message-ID: <
> Content-Type: text/plain;	charset="us-ascii"
> 
> Hi,
> I am using ABC kit for DAB-IHC experiment. But somehow, I don't feel it
> is a good choice since the background is kinda high. Is there anybody
> having some suggestions? Which kind of steptavidin-HRP are you using for
> DAB-IHC? From which company?
> 
> Thank you very much for your kind reply,
> 
> 
> Best,
> Karen
> 
> Karen Cai
> Research Scientist
> Prosci Incorporated
> (858) 513-2638 x 204
> (858) 513-2692 Fax
>  <http://www.prosci-inc.com> www.prosci-inc.com
> 
> 
> 
> ------------------------------
> 
> Message: 2
> Date: Fri, 14 Aug 2009 14:02:37 -0600
> From: "Matthew Lunetta" <
> Subject: [Histonet] HT opening in Colorado
> To: <
> Message-ID: <
> Content-Type: text/plain;	charset=US-ASCII
> 
> Hello Histo-Netters,
> 
> We have an HT job opening here in Longmont Colorado, located north of Denver and just NE of Boulder. Please apply through the website. I have attached the link. All new and experianced HT's please apply. Also please add my name to your application so we can track the responces from histo net.
> 
> 
> http://luh.jobscience.com/JsrApp/index.cfm?cmd=showPositionDetail&nextEvent=doSearchPositions&cobrandId=9000&masterId=luh001&accountId=9F3D0B3C-E71A-17E7-69B649D49CA1C88F&positionId=511568&urlArgList=c2VhcmNoVHlwZT1xdWljayZzdGFydD0xJmNvdW50PTUwJmRlcHRJZD0mam9iSWQ9JmtleXdvcmQ9&bid=1297
> 
> If the link does not work the web site is www.luhcares.org
> 
> Thanks
> Matt Lunetta
> BS, HT (ASCP)
> 
> 
> 
> 
> ------------------------------
> 
> Message: 3
> Date: Fri, 14 Aug 2009 16:20:34 -0400
> From: "Woodward, Denise" <
> Subject: [Histonet] unsubscribe
> To: "histonet <@t> lists.utsouthwestern.edu"
> 	<
> Message-ID:
> 	<
> 	
> Content-Type: text/plain; charset="us-ascii"
> 
> 
> 
> 
> ------------------------------
> 
> Message: 4
> Date: Fri, 14 Aug 2009 13:27:50 -0700
> From: "Konni Black" <
> Subject: [Histonet] Job Opening 
> To: <
> Message-ID: <
> Content-Type: text/plain;	charset="Windows-1252"
> 
> Hello All,
> 
> There is a new histology lab opening in St. Augustine, Florida. Beautiful area on the Palm Coast. 
> Great pay and working conditions. Extremely flexible hours. In fact, create your own schedule! 
> 
> Contact me for details or send resume to kblack <@t> digestivelth.com Don't miss this ground floor opportunity.
> 
> Konni Black
> 
> ------------------------------
> 
> Message: 5
> Date: Fri, 14 Aug 2009 14:47:54 -0700
> From: Tasha Fraser <
> Subject: [Histonet] Shandon Excelsior Tissue Processor
> To: "histonet <@t> lists.utsouthwestern.edu"
> 	<
> Message-ID:
> 	<
> 	
> Content-Type: text/plain; charset="us-ascii"
> 
> Would love to get some feedback hopefully both good and bad in regards
> to the Shandon Excelsior Tissue Processor.  Looking to purchase a new
> tissue processor and I already have a Tissue-Tek VIP 5.  I'm going to
> Demo a Shandon Excelsior and probably a Leica ASP300 S.  I do love the
> Tissue-Tek VIP's, but don't feel the need to demo the VIP6.  Any
> feedback on any of these processors would be greatly appreciated!
> 
> 
> 
> Tasha Fraser, HT (ASCP)
> 
> Olympic Medical Center
> 
> Port Angeles, WA 
> 
> 
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> ------------------------------
> 
> Message: 6
> Date: Fri, 14 Aug 2009 18:29:25 -0400
> From: Amos Brooks <
> Subject: [Histonet] Can DAB block an epitope??
> To: emerald_lake77 <@t> yahoo.com, histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> 	<
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> 
> Hi Gustave,
>     I do this frequently, and it works great with some caveats. First it is
> best to use a *very* red chromogen (if you choose to use red). I have not
> had a lot of luck with AEC the fast red in the DAKO alk phos kit looks great
> against DAB.
>     Colocalization of antigens can be tricky. In this case
> immunofluoresfence really is the way to go. Biocare Medical has some great
> Fluorescent secondaries. Colocalized chromogens just look muddy and not very
> good. The DAB doesn't really 'block' the epitope, but ut does make it a heck
> of a lot harder to see.
> 
> Good Luck,
> Amos
> 
> 
> Message: 19
> Date: Fri, 14 Aug 2009 08:37:45 -0700 (PDT)
> From: GT Hebert <
> Subject: [Histonet] Can DAB block an epitope??
> To: Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <
> Content-Type: text/plain; charset=iso-8859-1
> 
> Hello all,
> 
> I was hoping to run a chromogenic immuno to check if two proteins
> colocalize.
> 
> Since both antibodies are made in rabbit, I was going to stain one and
> visualize with DAB and then heat retrieve (pressure or steam) to drop off
> the first antibody and restain with my second rabbit antibody using a
> different color to visualize.
> 
> How likely, if at all will the DAB precipitate block / mask the epitope of
> my second antibody if indeed they are in a similar location?
> 
> Any info would be greatly appreciated.
> 
> Thank you!
> 
> Regards.
> 
> Gustave
> 
> Gustave T. Hebert
> Research Scientist I
> Metabolic Disease Research
> Wyeth Research
> 200 CambridgePark Drive
> Cambridge, MA 02140
> 
> 
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> End of Histonet Digest, Vol 69, Issue 15
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