[Histonet] streptavidin peroxidase

[MKing]

Sigma E-2886 extravidin peroxidase, 1:1000 in 0.1 M PBS or sodium 
acetate on almost every IHC variation (fixation, species, primary) we 
do.  Supposed to be less nonspecific binding than streptavidin.

Mike King
UF Pharmacology & Therapeutics

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Message: 27
Date: Fri, 14 Aug 2009 09:56:56 -0700
From: "Karen Cai" <kcai <@t> prosci-inc.com>
Subject: [Histonet] Need help on Streptavidin-HRP conjugate for IHC
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000701ca1d00$3b8b7080$7a01a8c0 <@t> mic>
Content-Type: text/plain;	charset="us-ascii"

Hi,
I am using ABC kit for DAB-IHC experiment. But somehow, I don't feel it
is a good choice since the background is kinda high. Is there anybody
having some suggestions? Which kind of steptavidin-HRP are you using for
DAB-IHC? From which company?

Thank you very much for your kind reply,


Best,
Karen

Karen Cai
Research Scientist
Prosci Incorporated
(858) 513-2638 x 204
(858) 513-2692 Fax
  <http://www.prosci-inc.com> www.prosci-inc.com