[Histonet] Freezing in Hexane

[Della Speranza, Vinnie]

Hi Dorothy,
I've not used hexane. I think 2-methly butane is more commonly used if you are, as I suspect, snap freezing in a solvent chilled in liquid nitrogen. The solvent just needs to have a low freezing point. 2-methyl butane freezes at about - 150 degrees C. I do not know if hexane has a lower freezing point but you can determine that with an internet search.

My point in all of this is that you may have other choices for snap freezing if you can't get an answer to your original question re hexane as long as you are able to freeze the needed temperature.

Vinnie Della Speranza
Manager for Anatomic Pathology Services
165 Ashley Avenue Suite 309
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Traczyk7 <@t> aol.com
Sent: Wednesday, August 12, 2009 12:47 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Freezing in Hexane

I have a procedure that refers to freezing a tissue sample in chilled  
Hexane.  When I look in a chemical catalogue I find there are several  formulas 
listed.  Any direction on which one to use would be greatly  appreciated.  
Thank you.
Dorothy
 
Dorothy Murphy Traczyk
Murphy-Traczyk & Associates LLC
PO Box 602
Point Pleasant, NJ 08742
_dorothy <@t> mtahistology.com_ (mailto:dorothy <@t> mtahistology.com) 
www.mtahistology.com
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