[Histonet] re: BD CD31

[PALMER Jason (SVHM)]

Actually we have been using BD CD31 on mouse tissue with routine FFPE fixation with good results for a while now - Zn fixation not needed.  Dako proteinase K 8 min, primary at 1:150, biotinylated secondary then Vector ABC with DAB substrate works well for us.

Cheers,  Jason

Jason Palmer
Histology Laboratory Coordinator
Bernard O'Brien Institute
42 Fitzroy St, Fitzroy Victoria 3065
Australia
tel +61 3 9288 4018
fax +61 3 9416 0926
email: jason.palmer <@t> svhm.org.au


------------------------------

Message: 4
Date: Mon, 10 Aug 2009 15:14:05 -0400
From: "Amy Porter" <portera <@t> msu.edu>
Subject: Re: [Histonet] CD31 working protocol
To: "Zerfas, Patricia \(NIH/OD/ORS\) [E]" <zerfasp <@t> ors.od.nih.gov>,
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <98811FBE01C548AF99A3C982238F8D2B <@t> histolab>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
        reply-type=original

BD Biosciences has a great rodent CD31 for mouse and rat - however you need
to use their Zinc Fixative.  It is well worth the results!!
Amy S. Porter, HT (ASCP) QIHC
Investigative HistoPathology Laboratory - Supervisor
2201 Biomedical Physical Sciences Bldg.  Rm #2133
East Lansing, MI  48824-3320
Phone:  (517) 884-5026
Fax:  (517) 432-1368
Email:  portera <@t> msu.edu
Web:  www.humanpathology.msu.edu<http://www.humanpathology.msu.edu>
----- Original Message -----
From: "Zerfas, Patricia (NIH/OD/ORS) [E]" <zerfasp <@t> ors.od.nih.gov>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, August 10, 2009 2:50 PM
Subject: [Histonet] CD31 working protocol


Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies.
______________________________________________________________________
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email 
______________________________________________________________________