[Histonet] IHC - p53 analysis

Spees, Colleen Colleen.Spees <@t> osumc.edu
Tue Aug 11 12:25:07 CDT 2009

I am a Graduate Fellow working with access to Image Pro Plus (4.5.1) for
analyzing human prostate tissues - staining for p53.  The lab folks that were
experienced in this area have since moved on, so I'm starting from scratch
and had a few questions:

1.       I see that Image Pro Plus has released version 7.0.  I'm wondering
if this expensive upgrade is worth it for p53 IHC analysis or if anyone has
any experience with IPP.

2.       I understand that analysis for p53 IHC is quite challenging.  I
wondered if anyone had any general insights they could share - other (but not
too expensive) software that would be better to use; preference for hand
counting vs. computer counting; etc.

3.       Other references or resources that could further enlighten me in
this area or other points I need to consider.


Colleen Spees

Medical Dietetics 

School of Allied Medical Professions

The Ohio State University

306 Atwell Hall

453 West Tenth Avenue

Columbus, Ohio 43210-1234

colleen.spees <@t> osumc.edu <mailto:colleen.spees <@t> osumc.edu> 


614-266-9234 (c)



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