[Histonet] CD31 working protocol

[dusko trajkovic]

Hi Patricia,
I use an antibody from Spring Biosciences Cat#M3384 to stain our human xenograft tumors as well as human tissue. Antibody will also stain mouse tissue. Very nice clean crisp stain. At least when we use it on our Bond IHC stainiers.
Thanks and let me know if you need more information.
dusko




________________________________
From: "Zerfas, Patricia (NIH/OD/ORS) [E]" <zerfasp <@t> ors.od.nih.gov>
To: dusko trajkovic <dunatrsd <@t> sbcglobal.net>
Sent: Monday, August 10, 2009 12:07:29 PM
Subject: RE: [Histonet] CD31 working protocol


Dusko,
            I am using mouse tissue.
 
Patricia Zerfas
National Institutes of Health
Building 28A, Room 112
28 Library Drive
Bethesda, MD   20892
ph:   (301) 496-4464
fax:  (301) 402-1068
 

________________________________

From:dusko trajkovic [mailto:dunatrsd <@t> sbcglobal.net] 
Sent: Monday, August 10, 2009 3:06 PM
To: Zerfas, Patricia (NIH/OD/ORS) [E]
Subject: Re: [Histonet] CD31 working protocol
 
What species are you trying to stain?
thanks
Dusko
 

________________________________

From:"Zerfas, Patricia (NIH/OD/ORS) [E]" <zerfasp <@t> ors.od.nih.gov>
To: " histonet <@t> lists.utsouthwestern.edu " < histonet <@t> lists.utsouthwestern.edu >
Sent: Monday, August 10, 2009 11:50:50 AM
Subject: [Histonet] CD31 working protocol

Dear Listservers,
            I have tried MANY protocols and I am unable to obtain a working protocol for the CD31 antibody.
            I have tried both the CD31 rat monoclonal and a CD31 rabbit polyclonal.

Thanks in advance for your help.

Patricia Zerfas
National Institutes of Health
Building 28A, Room 112
28 Library Drive
Bethesda , MD   20892
ph:  (301) 496-4464
fax:  (301) 402-1068

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