[Histonet] Good Fibrin stain with Movat's?

[Galbraith, Joe]

Judy:

Amos has a good suggestion.

Below is a link to a Movat procedure from Penn, we discontinued our
clinical Movat procedure due to lack of use (though it is a great
stain).  In the attached link, note the comments about the wash and
differentiation steps.  The Movat is nice in that it allows you to
differentiate elastic, fibrin and collagen.  Check after the alcian blue
to see if the fibrin is already stained blue.  Adjust concentrations and
timing if necessary.  Ensure that the alkaline alcohol is completely
removed.  Good luck.

http://www.med.upenn.edu/mcrc/histology_core/movat.shtml

Joe Galbraith
University of Iowa
joseph-galbraith <@t> uiowa.edu


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amos
Brooks
Sent: Sunday, August 09, 2009 6:03 AM
To: juditw <@t> u.washington.edu; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Good Fibrin stain with Movat's?

Hi,
   Have you tried a Sirius red (in sturated picric acid) with a
Weigart's
hamatoxylin for the Nucleii? I usually don't bother counterstaining the
nucleii  with this stain, but I suppose it would work. You wouuld
probably
want to do the hematoxylin after the Sirius red since it is an acidic
solution and would bleach out the hematoxylin otherwise. While this
isn't a
Movat's I think it might do the trick for you.

Amos

Message: 2
Date: Fri, 7 Aug 2009 10:21:11 -0700 (PDT)
From: "Judith L. Williams" <juditw <@t> u.washington.edu>
Subject: [Histonet] Good Fibrin stain with Movat's?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
       <Pine.LNX.4.43.0908071021110.27227 <@t> hymn33.u.washington.edu>
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Greetings to all histonetters- I have a pathologist that wants to show
fibrin in lung. She wants also black nuclei and we tried  a Movat's to
look
at fibrin. The fibrin in the lung is showing up light blue not red!
Any suggestions on how to show fibrin distinctly, with black nuclei?
thanks!
Judy
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