[Histonet] What do you use for decolorizing after Hematox.

[Michelle MacVeigh-Aloni]

Hi all,

In my H&E staining I always used 1% acid alcohol. (Working with conc. HCl is not fun!) 

There is the Orth's solution available already made, but it is 3%. Do you use that? How do you use it?

What do you use in your regulat H&E stain? I am using it on an old Jung AUTOSTAINER.

Michelle
Research Specialist 
USC Keck School of Medicine