[Histonet] Eosin Bleeding

[Monfils, Paul]

Can't say for sure but eosin is very sensitive to pH, and will rinse out rapidly in any solution that is basic, even slightly so, like tap water.  Nair contains calcium hydroxide, which is a stong base. If that compound were not thoroughly rinsed out of the tissue before processing, I wouldn't be surprised if it interfered with eosin binding.

> ----------
> From: 	histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Goins, Tresa
> Sent: 	Tuesday, August 4, 2009 5:11 PM
> To: 	histonet <@t> lists.utsouthwestern.edu
> Subject: 	[Histonet] Eosin Bleeding 
> 
> We very occasionally have a single slide out of a group of 100 or more where the eosin will bleed after coverslipping.  Does anyone know if this is associated with a particular tissue type or treatment with Nair to soften keratin?
> 
> Thanks,
> 
> Tresa
> Veterinary Diagnostic Lab
> Bozeman, MT
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
>