[Histonet] Re: Xylene substitutes (UNCLASSIFIED)

Gladney, Diane C Ms CIV USA MEDCOM MACH diane.gladney <@t> us.army.mil
Wed Aug 5 15:12:24 CDT 2009

Classification:  UNCLASSIFIED 
Caveats: NONE

I have 2 Leica ASP 300 tissue processors. I use Thermo Scientific Shandon Xylene Substitute (formally known as Histosolv). I have used this for many years with excellent results.  I have never had any problems using this in the processor. We had 2 very old VIP tissue processors before we got the Leica processors and used Histosolv in those processors also. I have never heard of Sub-X.  

Diane C. Gladney, HT (ASCP)
Supervisor, Anatomical Pathology 
Moncrief Army Community Hospital
Dept. of Pathology
4500 Stuart St.
FT. Jackson, SC  29207

Email:  diane.gladney <@t> amedd.army.mil

Phone:  803-751- 2530 
FAX:     803-751-7829
DSN:    734-2530

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robert Richmond
Sent: Wednesday, August 05, 2009 4:03 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Xylene substitutes

William (Bill) O'Donnell, HT (ASCP) QIHC, Lead Histologist, Good
Samaritan Hospital, Kearney, Nebraska, notes that Clear-Rite 3 is at
least temporarily unavailable, and he is looking for a substitute.
(Anybody know what's going on with Clear-Rite 3?)

Clear-Rite 3 is an aliphatic (rather than a limonene). Aliphatics
can't always be substituted for each other - they are chemically
different from each other - each has its own flammability
characteristics (flash point) and (if you're recovering solvent using
a spinning band still) its own distillation routine. If you change,
you may want to stay with the product you change to. Your purchasing
people need to be aware that they are not to change brands on you.

I just worked in a lab that has a Leica ASP300S processor (which they
like, by the way). The people there have been told that they may use
only one particular aliphatic with it, one I hadn't heard of before:

aliphatic hydrocarbon isoparaffinic oil
CAS 6472-48-9    DOT petroleum distillates, NOS, 3
class 3 flammable liquid
Flash point 106 F (41 C)

Anybody know if this is actually the case?

Bob Richmond
Samurai Pathologist
Knoxville TN

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