[Histonet] Paraffin Sections

thecitan <@t> yahoo.com thecitan <@t> yahoo.com
Tue Aug 4 17:09:22 CDT 2009

You running particularly small specimens like derm? You may need to decrease time in alcohol. Adding a little ammonia water to your ice bath and soaking after facing the block may help too.
------Original Message------
From: Kathleen Roberts
Sender: histonet-bounces <@t> lists.utsouthwestern.edu
To: Nagappan, Peri
Cc: Histonet
Subject: Re: [Histonet] Paraffin Sections
Sent: Aug 4, 2009 1:40 PM


Sounds like poor infiltration to me.  What kind of tissue are we talking 
about, and what processing program did you use?

Principal Lab Technician
Neurotoxicology Labs
Dept of Pharmacology & Toxicology
Rutgers, the State University of NJ
41 B Gordon Rd
Piscataway, NJ 08854

Nagappan, Peri wrote:

>Hi Histonetters,
>When I cut the paraffin sections in the microtome, I am not getting the whole sections intact, rather some portion in the middle of the sections are brittle. But the paraffin portion surrounds the tissue is smooth, nice and intact. 
>Thanks for your suggestion and help.
>pnagappan <@t> cau.edu <mailto:pnagappan <@t> cau.edu> 
><mailto:pnagappan <@t> cau.edu>  
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