[Histonet] Paraffin Sections

Kathleen Roberts kgrobert <@t> rci.rutgers.edu
Tue Aug 4 15:40:20 CDT 2009


Peri,

Sounds like poor infiltration to me.  What kind of tissue are we talking 
about, and what processing program did you use?

Kathleen
Principal Lab Technician
Neurotoxicology Labs
Dept of Pharmacology & Toxicology
Rutgers, the State University of NJ
41 B Gordon Rd
Piscataway, NJ 08854

Nagappan, Peri wrote:

>Hi Histonetters,
>
> 
>
>When I cut the paraffin sections in the microtome, I am not getting the whole sections intact, rather some portion in the middle of the sections are brittle. But the paraffin portion surrounds the tissue is smooth, nice and intact. 
>
> 
>
>Thanks for your suggestion and help.
>
> 
>
>Peri 
>
>pnagappan <@t> cau.edu <mailto:pnagappan <@t> cau.edu> 
>
><mailto:pnagappan <@t> cau.edu>  
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