[Histonet] Golgi stain

[MKing]

Frauke,

There are some published methods for doing Golgi on brain sections:

Freund TF, Somogyi P. The section-Golgi impregnation procedure. 1. 
Description of the method and its combination with histochemistry after 
intracellular iontophoresis or retrograde transport of horseradish 
peroxidase. Neuroscience. 1983 Jul;9(3):463-74.

Somogyi P, Freund TF, Wu JY, Smith AD. The section-Golgi impregnation 
procedure. 2. Immunocytochemical demonstration of glutamate 
decarboxylase in Golgi-impregnated neurons and in their afferent 
synaptic boutons in the visual cortex of the cat. Neuroscience. 1983 
Jul;9(3):475-90.

Bolam JP, Ingham CA, Smith AD. The section-Golgi-impregnation
procedure--3. Combination of Golgi-impregnation with enzyme
histochemistry and electron microscopy to characterize
acetylcholinesterase-containing neurons in the rat neostriatum.
Neuroscience. 1984 Jul;12(3):687-709.

Gabbott PL, Somogyi J. The 'single' section Golgi-impregnation 
procedure: methodological description. J Neurosci Methods. 1984 
Sep;11(4):221-30.

Harris KM, Cruce WL, Greenough WT, Teyler TJ. A Golgi impregnation 
technique for thin brain slices maintained in vitro. J Neurosci Methods. 
1980 Aug;2(4):363-71.

Spacek J. Dynamics of the Golgi method: a time-lapse study of the early 
stages of impregnation in single sections. J Neurocytol. 1989 
Feb;18(1):27-38.

Moss TL, Whetsell WO. Techniques for thick-section Golgi impregnation of 
formalin-fixed brain tissue. Methods Mol Biol. 2004;277:277-85.

I don't remember if any of these discuss re-staining, but if you don't 
have any silver chromate crystals in the specimens it probably can't 
hurt to return them to the chromation step.  Tricky business getting 
consistent staining on sections in any case.  You might still be able to 
use a Cox alternative.  I've had good luck with Gibb and Kolb's method 
on some thick sections although it was described for whole brain (Gibb 
R, Kolb B. A method for vibratome sectioning of Golgi-Cox stained whole 
rat brain. J Neurosci Methods. 1998 Jan 31;79(1):1-4.).

Good luck,
Mike King
UF Pharmacology & Therapeutics

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Message: 20
Date: Tue, 04 Aug 2009 17:51:17 +0200
From: "Dr. Frauke Neff" <nefff <@t> staff.uni-marburg.de>
Subject: [Histonet] golgi stain- please help
To: histonet <@t> lists.utsouthwestern.edu
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Dear Histonetters,
I was performing a golgi stain on mouse brains and my treated animal
group gave nice results but the untreated control group did not show
any staining at all (it looks like the staining didn't work).
Does anyone know / have an idea, if I could repeat the staining on
these vibratome sections?
I read something about "deimpregnation" of golgi stained slides, but
found no protocol and I'm not sure if I can perform another "fresh"
golgi stain with these slides.

Any suggestion is welcome!!!

Thanks to all of you,
Frauke