[Histonet] golgi stain- please help

[John Kiernan]

Golgi staining and related methods are done on whole, fresh pieces of brain tissue, not on sections. There are some techniques for fixed specimens. Instructions can be found in books of neurohistological technique; older ones often have plenty of technical tips. A good start would be: Santini, M., ed. (1975). Golgi Centennial Symposium: Perspectives in Neurobiology. New York: Raven Press.
 
John Kiernan
Anatomy, UWO
London, Canada
= = =
----- Original Message -----
From: "Dr. Frauke Neff" <nefff <@t> staff.uni-marburg.de>
Date: Tuesday, August 4, 2009 11:52
Subject: [Histonet] golgi stain- please help
To: histonet <@t> lists.utsouthwestern.edu

> Dear Histonetters,
> I was performing a golgi stain on mouse brains and my treated 
> animal  
> group gave nice results but the untreated control group did not 
> show  
> any staining at all (it looks like the staining didn't work).
> Does anyone know / have an idea, if I could repeat the staining 
> on  
> these vibratome sections?
> I read something about "deimpregnation" of golgi stained slides, 
> but  
> found no protocol and I'm not sure if I can perform another 
> "fresh"  
> golgi stain with these slides.
> 
> Any suggestion is welcome!!!
> 
> 
> Thanks to all of you,
> 
> 
> Frauke
> 
> 
> 
> 
> 
> 
> 
> 
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