[Histonet] tissue submission question

[Tony Henwood]

Laurie,

It is a little fiddly but as long as you keep the animal frozen, you can
dissect out the muscle, quickly place it on some OCT on a chuck and
freeze the chuck. Try to not let the tissue thaw.

How big is the animal?

You could do this on the freezing plate of the embedding centre or on
the cold shelf in the cryostat.
It is important throughout this process to not let the tissue thaw too
much.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
laurie <@t> conxis.com
Sent: Saturday, 1 August 2009 6:58 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] tissue submission question


Happy Friday everyone.

I have an investigator that wants to submit leg muscle from a rat for
IHC staining.  The problem is they did not dissect the muscle out after
euthanizing the animal, they just froze the whole animal.  We do not
have a sliding microtome so that we can just "slice" the animal up ( yes
the investigator called it slicing.)  Any thoughts out there?

No this is not a Fun Friday joke :-(

Have a great weekend!
Laurie

Laurie Popp, BA HT ( ASCP)


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