[Histonet] RE: mounting media for IHC

Hobbs, Carl carl.hobbs <@t> kcl.ac.uk
Thu Apr 30 13:17:35 CDT 2009


Hi.
I use DPX from VWR for all of my std dye-based stainings (such as H&E, Alcian blue/PAS) and also for my DAB IHC: 500ml for 35UK£.
I note that the equivilent Zymed product, Histomount is a similar UK price.....but, for 15ml.
Perhaps I am wrong?
I have used Histomount and DPX and do not notice any difference to the stainings under the microscope.
carl


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Subject: Histonet Digest, Vol 65, Issue 51

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Today's Topics:

   1. Colorado Society of Histotechnology - Meeting May 8th & 9th
      in Colorado Springs (McGinley,John)
   2. NSH Call for Awards Applications/Nominations (Mitchell Jean A)
   3. Rolling sections (Pixley, Sarah (pixleysk))
   4. mounting media for IHC (Rashmil)
   5. MMR protein antibodies for Lynch Syndrome (Taylor, Jean)
   6. Reusing Citrate Antigen Retrieval Buffer (Jason McGough)
   7. Position In Florida (Alyssa Peterson)
   8. Re: Reusing Citrate Antigen Retrieval Buffer (TF)
   9. Re: mounting media for IHC ( TF )


----------------------------------------------------------------------

Message: 1
Date: Wed, 29 Apr 2009 13:20:27 -0600
From: "McGinley,John" <John.McGinley <@t> ColoState.EDU>
Subject: [Histonet] Colorado Society of Histotechnology - Meeting May
        8th & 9th in Colorado Springs
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <B7F026438BA66648A872804B07B904563F81F5409E <@t> EVS1.ColoState.EDU>
Content-Type: text/plain; charset="us-ascii"

Hi,

The Colorado Society of Histotechnology (CSH) meeting will be held at the Cheyenne Mountain Resort in Colorado Springs, May 8th and 9th, 2009. If you are interested in attending the meeting and have not registered, please visit our website at http://www.coloradohisto.org/2009/meeting.htm. Online registration is available and payment can made on the day of the meeting (check only. Sorry, no credit cards accepted at this time). Thank you and I look forward to seeing you at the meeting.

Regards,

John McGinley
CSH Secretary



------------------------------

Message: 2
Date: Wed, 29 Apr 2009 23:17:52 -0500
From: "Mitchell Jean A" <JMitchell <@t> uwhealth.org>
Subject: [Histonet] NSH Call for Awards Applications/Nominations
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <2108AECB05DBFF48A9C436A792155740010C349D <@t> UWHC-MAIL03.uwhis.hosp.wisc.edu>

Content-Type: text/plain;       charset="iso-8859-1"

Last Call for Nominations/Applications:



You still have time to make the NSH 2009 Awards/Scholarship May 1st deadline!  Each year, NSH recognizes the commitment and achievements of individuals in the histology profession, spotlights students and their achievements, and applauds the innovation of members dedicated to advancing our science. We invite you to apply and or nominate a professional for the 2009 NSH Awards and Scholarship Program. Please review the detailed descriptions of each Award and their respective criteria at www.nsh.org and then nominate the deserving professional.



Be sure to submit applications by FRIDAY; the nomination deadline of May 1, 2009.



Jean Mitchell - Chair, 2009 NSH Awards Committee




------------------------------

Message: 3
Date: Thu, 30 Apr 2009 09:48:05 -0400
From: "Pixley, Sarah (pixleysk)" <PIXLEYSK <@t> UCMAIL.UC.EDU>
Subject: [Histonet] Rolling sections
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <E13205C9CB112A44B029C1C3F20EB8FF335AF75410 <@t> UCMAILBE5.ad.uc.edu>
Content-Type: text/plain; charset="us-ascii"

At one time, when we were using knives (not disposable blades), and we were using a roller plate, I found that the Shandon (now Thermo Scientific) M1 Embedding Matrix: http://www.thermo.com/com/cda/product/detail/1,1055,21902,00.html appeared to cause less rolling than OCT. You could try that. (However, now my lab is now using OCT, with disposable blades, without a roller plate, and they are not having difficulties cutting rat brains.)

Another thing to check is to make sure all the settings are correct, as in previous responses: temperature, thickness, etc. Also, be sure that the knife angle is adjusted perfectly for your tissue and your temperature. Subtle changes in angle can help sectioning in many ways. Finally, if static electricity is a problem, you can try removing gloves (if you are not trying to be RNase free). Gloves can sometimes, depending on the type, increase static.
Best,
Sarah




------------------------------

Message: 4
Date: Thu, 30 Apr 2009 07:40:34 -0700 (PDT)
From: Rashmil <rashmil_histotechnology <@t> yahoo.com>
Subject: [Histonet] mounting media for IHC
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <896581.68977.qm <@t> web59307.mail.re1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1



Hi

Is
there a mounting solution that is better than another for IHC slides? I
am using Histomount from ZYMED. And have no problems with it. But I need
to order some more and was wondering if there was one product that was
better than the other? ?I was especially interested to hear about
Ecomount from Biocare.

Thanks,
Rashmil




------------------------------

Message: 5
Date: Thu, 30 Apr 2009 09:47:42 -0500
From: "Taylor, Jean" <jtaylor <@t> meriter.com>
Subject: [Histonet] MMR protein antibodies for Lynch Syndrome
To: "'ihcrg <@t> googlegroups.com'" <ihcrg <@t> googlegroups.com>,
        "'histonet <@t> lists.utsouthwestern.edu'"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <466B666475DE6547BBB0641E540A4BB504874767D1 <@t> EXVS1.meriter.com>
Content-Type: text/plain; charset="us-ascii"

Hi Everyone,

I'm looking for information about MLH1, MSH2, MSH6 and PMS2 and any other IVD antibody that labs are using to identify Lynch Syndrome. Would appreciate any information regarding antibody source, retrieval methods, dilutions, etc...

Thank you!

Jean Taylor, HT(ASCP) QIHC
IHC Tech
Meriter Health Services
36 S. Brooks St.
Madison, WI 53715


------------------------------

Message: 6
Date: Thu, 30 Apr 2009 09:09:10 -0600
From: "Jason McGough" <jmcgough <@t> clinlab.com>
Subject: [Histonet] Reusing Citrate Antigen Retrieval Buffer
To: <ihcrg <@t> googlegroups.com>,   <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BDEKKAEJKLLOFEDGDEBEAEFBCCAA.jmcgough <@t> clinlab.com>
Content-Type: text/plain;       charset="us-ascii"

We are looking into using our .01M Citrate Antigen Retrieval Buffer in our
Dako PT Link for Antigen Retrieval on our IHC stains. The question that we
are wondering about is anybody reusing this solution for multiple times? If
so, how many times? What is your dilution? Like the High pH Antigen
Retrieval solution from Dako, it is recommended to use up to 3 time before
replacement.

We are having several problems with the High pH antigen retrieval solution
from Dako and want to try Citrate, since that is what we have been using for
many years with great results. We previously used the Pascal Pressure Cooker
from Dako for Antigen Retrieval but now have the PT Link. Thank you in
advance for your replies.

Jason McGough HT(ASCP)
Account Representative - Anatomic Pathology
Clinical Laboratory of the Black Hills
2805 5th Street Suite 210
Rapid City, SD 57701
605-343-2267 Ext 127
605-718-3779 (Fax)
jmcgough <@t> clinlab.com






------------------------------

Message: 7
Date: Thu, 30 Apr 2009 11:26:13 -0400
From: Alyssa Peterson <alyssa <@t> alliedsearchpartners.com>
Subject: [Histonet] Position In Florida
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <bbc6db3a0904300826g555650a8gf6c6aee2aefd6319 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Hello,



My name is Alyssa Peterson, and I am the director of lab/pathology
recruitment at Allied Search Partners, and I wanted to follow up with you in
reference to a full time/permanent job opportunity for a talented
histotechnologist. The position is in* Auburndale, Fl area. **If this is not
the job for you, however, you are interested in exploring new opportunities
please send me a copy of your updated resume and what you are looking for*.
Also, we offer to pay you up to $1000 for any referral that we place in a
position, so please forward this to anyone who seems fit for this position
and have them contact me right away!



*Position: Histotechnologist*

* *

*Shift*

*Full time, day shift*

* *

*Job Description:*

Responsible for performance of specimen processing, embedding, cutting,
routine and special histologic staining, frozen sectioning and mounting of
preparations from surgical and autopsy materials for microscopic
interpretation by the Pathologist.  In addition, measures and describes
needle core biopsies of the breast, liver, and all endoscopy biopsies.
Assists Pathologist by measuring, apportioning muscle biopsies; sural nerve
biopsies, and needle core biopsies of the kidney.  Implements and tests new
techniques and procedures.  The Histotechnologist identifies tissue
structures, cell components, and their staining characteristics, relating
them to physiological functions, making judgments concerning the results of
quality control measures.  Also prepares fresh unfixed tissue for
transporting to a reference lab.

Requirements & Working Conditions

?         High School Diploma Required.

?         Completion of structured training and education in Histology.

?         Licensed by the State of Florida as a Histology Technologist.
ASCP certification preferred.

?         Ability to stand and walk for approximately twenty percent of work
time when preparing tissues, and ability to lift, carry and manipulate
solvents/solvent containers, supplies, or equipment to a maximum of 25 lbs.

?         Is on-call occasionally or adjusts work schedule to respond for
frozen sections.

?         Works in a laboratory histology section with frequent (up to
thirty percent of work time) exposure to unpleasant odors, chemicals and
bio-hazardous specimens.

?         Potential exposure to cuts on hands and fingers from microtome
knives but hazards are limited when established handling procedures are
followed.




--
Alyssa Peterson
Allied Search Partners
O: 770.621.2639 ext. 4
F: 770.621.2640


------------------------------

Message: 8
Date: Fri, 1 May 2009 00:24:12 +0800
From: "TF" <tifei <@t> foxmail.com>
Subject: Re: [Histonet] Reusing Citrate Antigen Retrieval Buffer
To: "Jason McGough" <jmcgough <@t> clinlab.com>,     "ihcrg <@t> googlegroups.com"
        <ihcrg <@t> googlegroups.com>,       "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <200905010024074693324 <@t> foxmail.com>
Content-Type: text/plain;       charset="gb2312"

We never tired that but I think you should not do that.
For the sake of the 2nd batch of sections; also, the solution pH, concentration, ion strength after boiling should have changed/


2009-05-01



TF



???????? Jason McGough
?????????? 2009-04-30  23:15:31
???????? ihcrg <@t> googlegroups.com; histonet <@t> lists.utsouthwestern.edu
??????
?????? [Histonet] Reusing Citrate Antigen Retrieval Buffer

We are looking into using our .01M Citrate Antigen Retrieval Buffer in our
Dako PT Link for Antigen Retrieval on our IHC stains. The question that we
are wondering about is anybody reusing this solution for multiple times? If
so, how many times? What is your dilution? Like the High pH Antigen
Retrieval solution from Dako, it is recommended to use up to 3 time before
replacement.
We are having several problems with the High pH antigen retrieval solution
from Dako and want to try Citrate, since that is what we have been using for
many years with great results. We previously used the Pascal Pressure Cooker
from Dako for Antigen Retrieval but now have the PT Link. Thank you in
advance for your replies.
Jason McGough HT(ASCP)
Account Representative - Anatomic Pathology
Clinical Laboratory of the Black Hills
2805 5th Street Suite 210
Rapid City, SD 57701
605-343-2267 Ext 127
605-718-3779 (Fax)
jmcgough <@t> clinlab.com
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 9
Date: Fri, 1 May 2009 00:25:10 +0800
From: " TF " <tifei <@t> foxmail.com>
Subject: Re: [Histonet] mounting media for IHC
To: " rashmil_histotechnology " <rashmil_histotechnology <@t> yahoo.com>,
        " histonet " <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <200905010025053558870 <@t> foxmail.com>
Content-Type: text/plain;       charset="utf-8"

Hi, we are using DAKO fluorescent mounting medium.
We also have DAPI-mouting medium so that you dont have to stain for DAPI/hoechest.


2009-05-01



TF



???????????? Rashmil
??????????????? 2009-04-30  22:44:48
???????????? histonet
?????????
????????? [Histonet] mounting media for IHC

Hi
Is
there a mounting solution that is better than another for IHC slides? I
am using Histomount from ZYMED. And have no problems with it. But I need
to order some more and was wondering if there was one product that was
better than the other? ??? was especially interested to hear about
Ecomount from Biocare.
Thanks,
Rashmil

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Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

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