[Histonet] Destaining H&E slides

[Rene J Buesa]

You can destained eosin with diluted (1:20) bleach.
René J.

--- On Tue, 4/28/09, Kathleen Roberts <kgrobert <@t> rci.rutgers.edu> wrote:

From: Kathleen Roberts <kgrobert <@t> rci.rutgers.edu>
Subject: [Histonet] Destaining H&E slides
To: "'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu>
Date: Tuesday, April 28, 2009, 3:29 PM

I searched the archives and found Gary Gill's method for destaining H&E
slides, and for destaining eosin he calls for 1.5% NH4OH in 70% ethanol....the
problem is, I don't have any NH4OH in the lab.  Is there another way to
fully destain eosin?

Thanks,
Kathleen
Principal Lab Technician
Neurotoxicology Labs
Dept of Pharmacology & Toxicology
Rutgers, the State University of NJ
41 B Gordon Rd
Piscataway, NJ 08854
(732) 445-6914
FAX (732) 445-6905
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