[Histonet] Cracked or shattered LN sections

[Greg Dobbin]

I am positive no freezing spray being used.
Greg

Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

"I find that the harder I work, the 
more luck I seem to have."
- Thomas Jefferson

>>> "Sebree Linda A" <LSebree <@t> uwhealth.org> 04/27/09 7:36 PM >>>
Greg,
 
Are you sure no one is using freezing spray when sectioning?  The effect
of freezing spray on the block would yield the appearance you describe.

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Greg Dobbin
Sent: Mon 4/27/2009 2:04 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Cracked or shattered LN sections



Hi Folks,
Some lymph node sections (usually bigger ones-maybe almost as big as a
dime) have a shattered appearance on the block surface. Subsequently the
sections look like shattered glass or a mosaic of tissue pieces making
up the whole. I think this is happening at the embedding station from
trying to make sure that the tissue is flat in the mold (ie pressing the
tissue down). Has anyone else out there have any similar experience with
this problem? Is the solution as simple as cutting smaller pieces into
the cassette at the time of trimming?
Thanks.
Greg

Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

"I find that the harder I work, the
more luck I seem to have."
- Thomas Jefferson


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