[Histonet] AMMONIUM HYDROXIDE

[Sebree Linda A]

We've never seen that correlation Phyllis but that doesn't mean there isn't one.  Our ammonium hydroxide is used at 5% followed by chilling the block, then sectioning.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Phyllis Thaxton
Sent: Monday, April 27, 2009 9:02 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] AMMONIUM HYDROXIDE



Hi all,
  I looked in archives to see if I could find any info on this but did not. Remember, we are having a problem with some of the sections floating off during IHC. 
   If paraffin blocks are faced and then placed  in ammonium hydroxide before cutting the H&E, would this cause the sections to float off if the unstained sections that are cut in addition to the H&E's are also produced in this manner? Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL 


      
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