[Histonet] RE: Histonet Digest, Vol 65, Issue 44

Mon Apr 27 06:59:20 CDT 2009

Hi Bill,

You can look under California Society of Histology Meeting held in Millbrae
CA in May.

If you need any other information, please let me know.  Have a great day!

Best
Stella

Original Message:
-----------------
From: histonet-request <@t> lists.utsouthwestern.edu
histonet-request <@t> lists.utsouthwestern.edu
Date: Sun, 26 Apr 2009 13:08:30 -0400
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 65, Issue 44

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Today's Topics:

   1. paraffin embedding (Marc Shaeffer)
   2. Long term storage for IHC? (Mikael Niku)
   3. Re: Long term storage for IHC? ( TF )
   4. AW: [Histonet] Long term storage for IHC? (Gudrun Lang)
   5. Long term storage for IHC ? (Rene J Buesa)

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Message: 1
Date: Sat, 25 Apr 2009 10:15:50 -0700
From: "Marc Shaeffer" <mshaeffer <@t> cox.net>
Subject: [Histonet] paraffin embedding
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B7392E4768D3431D997FB5694D0C7FC5 <@t> youro0kwkw9jwc>
Content-Type: text/plain;	charset="us-ascii"

Don't forget to put the plastic cassette on top the mold prior to filling
and solidifying the paraffin.

------------------------------

Message: 2
Date: Sat, 25 Apr 2009 23:24:56 +0300
From: Mikael Niku <mikael.niku <@t> helsinki.fi>
Subject: [Histonet] Long term storage for IHC?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <49F37198.5030907 <@t> helsinki.fi>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Dear Histonetters,

what do you think is the best way to store formalin-fixed tissues for 
immunohistochemistry, long-term (several years)?

We are currently fixing overnight, then rinsing in buffer and storing in 
70% or 100% ethanol, +4C or -20C. However, I don't really KNOW what 
happens here and how the tissues will work after the years. I guess 
basically the formalin fixation will be at least somewhat reversed and 
after some time the tissues will be more like ethanol fixed. But are 
there better options? Long term storage in formalin will make IHC 
difficult. And this is about large numbers of tissue samples, only some 
of which will be actually used later, so we wouldn't like to process all 
of them to paraffin either.

With best regards,
Mikael Niku
University of Helsinki, Finland

------------------------------

Message: 3
Date: Sun, 26 Apr 2009 13:19:53 +0800
From: " TF " <tifei <@t> foxmail.com>
Subject: Re: [Histonet] Long term storage for IHC?
To: " Mikael Niku " <mikael.niku <@t> helsinki.fi>, " histonet "
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <200904261319483656380 <@t> foxmail.com>
Content-Type: text/plain;	charset="utf-8"

Hi, i embeded all of them in OCT and put them under -20.
To prevent water vapor leakage, use some parafilm to pack the OCT block or
seal it.
We tested this in samples harvested 5 years ago (2004-2009).

2009-04-26 

TF 

å‘ä»¶äººï¼š Mikael Niku 
å‘é€æ—¶é—´ï¼š 2009-04-26  04:31:05 
æ”¶ä»¶äººï¼š histonet 
æŠ„é€ï¼š 
ä¸»é¢˜ï¼š [Histonet] Long term storage for IHC? 

Dear Histonetters,
what do you think is the best way to store formalin-fixed tissues for 
immunohistochemistry, long-term (several years)?
We are currently fixing overnight, then rinsing in buffer and storing in 
70% or 100% ethanol, +4C or -20C. However, I don't really KNOW what 
happens here and how the tissues will work after the years. I guess 
basically the formalin fixation will be at least somewhat reversed and 
after some time the tissues will be more like ethanol fixed. But are 
there better options? Long term storage in formalin will make IHC 
difficult. And this is about large numbers of tissue samples, only some 
of which will be actually used later, so we wouldn't like to process all 
of them to paraffin either.
With best regards,
Mikael Niku
University of Helsinki, Finland
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 4
Date: Sun, 26 Apr 2009 12:46:50 +0200
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Subject: AW: [Histonet] Long term storage for IHC?
To: "'Mikael Niku'" <mikael.niku <@t> helsinki.fi>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <7224DDE4D9664677A8281EECFDDD9309 <@t> dielangs.at>
Content-Type: text/plain;	charset="iso-8859-1"

I guess the formalin-fixation will be reversed although in a very slow
manner in the 70% ethanol. And I think, overnight fixation depending on the
tissue size will give insufficient formalin-fixation. This will result in
ethanol-fixation in the 70% and especially in the 100% ethanol.
If you want long time storage in ethanol, be sure that formalin-fixation is
sufficient. 
In my opinion your regular immunhistoprotocol has also to be adapted after
long time storage (more than 2 months), no matter if in formaldehyd or
ethanol.
What is more effort? Storage and administration of large numbers of wet
tissue in refrigerator or processing to paraffin blocks and storage in
drawers?

Gudrun Lang

-----Ursprï¿½ngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Mikael
Niku
Gesendet: Samstag, 25. April 2009 22:25
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Long term storage for IHC?

Dear Histonetters,

what do you think is the best way to store formalin-fixed tissues for 
immunohistochemistry, long-term (several years)?

We are currently fixing overnight, then rinsing in buffer and storing in 
70% or 100% ethanol, +4C or -20C. However, I don't really KNOW what 
happens here and how the tissues will work after the years. I guess 
basically the formalin fixation will be at least somewhat reversed and 
after some time the tissues will be more like ethanol fixed. But are 
there better options? Long term storage in formalin will make IHC 
difficult. And this is about large numbers of tissue samples, only some 
of which will be actually used later, so we wouldn't like to process all 
of them to paraffin either.

With best regards,
Mikael Niku
University of Helsinki, Finland

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 5
Date: Sun, 26 Apr 2009 07:38:53 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: [Histonet] Long term storage for IHC ?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <863080.7096.qm <@t> web65704.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hi Mikael:
Long term storage ofï¿½formalin fixedï¿½tissue is usually never a good
option. If you keep them in NBF the antigenic sites could be so compromised
that their unmasking could prove to be unsuccessful.
In 70% EthOL they will macerate and, as you point out, could end as if they
were alcohol fixed. There are really 2ï¿½options:
1- process the tissues andï¿½ save the uncut blocks, or
2- select the most interesting piecesï¿½and fix them for 48 hours to assure
full fixation and after washing in PBS freeze them and keep themï¿½frozen
at -80ï¿½C. When the moment arises that you will need them, thaw and
process them. I think thatï¿½ you should go with cryoperservation. I am
attaching an article I wrote about formalin fixation.
Renï¿½ J.
ï¿½
Dear Histonetters,

what do you think is the best way to store formalin-fixed tissues for 
immunohistochemistry, long-term (several years)?

We are currently fixing overnight, then rinsing in buffer and storing in 
70% or 100% ethanol, +4C or -20C. However, I don't really KNOW what 
happens here and how the tissues will work after the years. I guess 
basically the formalin fixation will be at least somewhat reversed and 
after some time the tissues will be more like ethanol fixed. But are 
there better options? Long term storage in formalin will make IHC 
difficult. And this is about large numbers of tissue samples, only some 
of which will be actually used later, so we wouldn't like to process all 
of them to paraffin either.

With best regards,
Mikael Niku
University of Helsinki, Finland

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
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_______________________________________________
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------------------------------

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End of Histonet Digest, Vol 65, Issue 44
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