Re: [Histonet] Long term storage for IHC?
tifei <@t> foxmail.com
Sun Apr 26 00:19:53 CDT 2009
Hi, i embeded all of them in OCT and put them under -20.
To prevent water vapor leakage, use some parafilm to pack the OCT block or seal it.
We tested this in samples harvested 5 years ago (2004-2009).
发件人： Mikael Niku
发送时间： 2009-04-26 04:31:05
主题： [Histonet] Long term storage for IHC?
what do you think is the best way to store formalin-fixed tissues for
immunohistochemistry, long-term (several years)?
We are currently fixing overnight, then rinsing in buffer and storing in
70% or 100% ethanol, +4C or -20C. However, I don't really KNOW what
happens here and how the tissues will work after the years. I guess
basically the formalin fixation will be at least somewhat reversed and
after some time the tissues will be more like ethanol fixed. But are
there better options? Long term storage in formalin will make IHC
difficult. And this is about large numbers of tissue samples, only some
of which will be actually used later, so we wouldn't like to process all
of them to paraffin either.
With best regards,
University of Helsinki, Finland
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