[Histonet] paraffin embedding

[V. Neubert]

Take some paraffin into the mould, then grab your tissue, press it to 
the bottom, hold it and get onto cooling plate. Wait a few seconds for 
the paraffin to start getting solid, then fill up with hot paraffin. I 
use this "technique" everyday.


Ryan McAdams wrote:
> I am trying to embed adult rat aortas in paraffin for histology purposes.
> Do you have any recommendations on an optimal method to keep the aortas in a
> vertical position while the paraffin solidifies?  I am hoping to do
> transverse sectioning of the thoracic aortas to analyze wall thickness. 
>
>  
>
> Thanks,
>
>  
>
> Ryan
>
>  
>
> Ryan McAdams
>
> Assistant Professor of Pediatrics 
>
> Division of Neonatology University of Washington
>
> Box 356320 Seattle, WA 98195-6320
>
> Telephone: (206)-616-8246
>
>  
>
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