[Histonet] RE: ground meat samples

[Rittman, Barry R]

Roger
If I were you I would compress this into a block before fixation.
Can use paper up to wax but would not use paper covering in the embedded block it will make cutting too difficult.
Alternative is to use sausage skin as the covering and process, embed and cut with this.
Barry

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Charles, Roger
Sent: Thursday, April 23, 2009 12:21 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: ground meat samples

Sorry Histonetter's
But it is my Friday and wouldn't this method give new meaning to the term "sausage block." :) I wish Margaret all the luck in her quest and if it was me, I would try either or both methods.


Roger Charles
Microbiologist
Pennsylvania Veterinary Laboratory
2305 N Cameron St
Harrisburg, PA 17110
717-787-8808
rcharles <@t> state.pa.us

No trees were hurt in the sending of this email, However many electrons were severely inconvenienced!


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Perry, Margaret
Sent: Thursday, April 23, 2009 12:53 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] ground meat samples

We will be doing IHC on ground pork and would like tips on how to process and embed the samples.  Can you leave the processed sample in a paper biopsy bag and embed both the meat and the paper?  Will cutting the paper make the blade dull to quickly?  Is there anything else we can use to hold the tissue together?  We had thought to maybe use pig intestine and pack the meat into it to make it cut better.
Thank you in advance for your help.  Histonet has helped me out many times and I greatly appreciate it.


Margaret Perry HT (ASCP)
IHC Lab Manager Veterinary Science
Animal Disease Research and Diagnostic Lab
South Dakota State University
Box 2175 North Campus Drive
Brookings SD 57007

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet