[Histonet] Cloudy MMA embedded block

[ooi.ting.huay <@t> nhc.com.sg]

Hi, 

I am trying very hard to get a clear MMA embedded block. I am appreciate 
if there is any advice on doing it. I am using 100% MMA (liquid) and 0.5% 
Perkadox 16 (powder) to do the embedding. I have tried to embed the sample 
in different conditions. However, it seems to be extremely hard to get a 
clear embedded block. 

Below are the methods that I have tried.

1. Before embedding, bring the MMA and perkadox 16 to room temperature. 
After a few mix it by invertion, I aliquot the solution to the glass vials 
that contain samples or simply glass vials that without sample. I put the 
glass vials (both with and without sample) in 4 degree, room temperature 
and even 37degree oven. 

2. Mix the MMA and perkadox when it is cold, invert it a few times. 
Aliquot it to glass vials that contain sample or without sample. Put in 
4degree, room temperature and 37degree oven. 

I have also tried to speed up the polymerization process by using vacumm 
for a few samples...

Throughout all the sample that I have, I only manage to get a clear empty 
glass vial which I put in the 4degree for one month and a clear embedded 
sample which I put in 4 degree overnight and bring it out to room 
temperature. The rest of the glass vials (with or without sample) are 
cloudy. 

I have repeated the methods that I used to get a clear embedded block, I 
did not get any clear block (for both with and without sample). 

I am glad if there is any expert advice or guidance. We need a clear 
embedded sample. Thank you very much!



Regards,
Ooi 
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