[Histonet] BRDU, tetracycline and calcein IV in bone

[Walters, Katherine S]

This may be a question for Gayle, or someone else who specializes in
bone.  I will be doing a project involving the facial bones of 8
week-old rabbits.  We want to look at regeneration of bone in this area.
I have some experience with BRDU in mouse tissues. 
 My questions are:
1.)  Will decalcification affect the BRDU signal?
	If not, what is the best method for decalcification?
Approximately how long?
2.)  Do I need to use a ground section for tetracycline and calcein IV
or can I decalcify and paraffin embed?
3.)  What is the best fixation procedure?
4.)  Anything I need to know, but am too naive to ask?

Thanks for any information you can share!

Katherine Walters
Histology Director
Central Microscopy Research Facilities
85 Eckstein Medical Research Building
University of Iowa
Iowa City, Iowa 52242-1101

katherine-walters <@t> uiowa.edu
www.uiowa.edu/~cemrf







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