[Histonet] Question about floating sections off slides

[Victor Tobias]

Jennitfer,

Found this in the archives from "Joe The Toe".

I remember the procedure. 1. Take off the cover slip and soak in xylene 
or substitute for 15 minutes. 2. Apply enough Mount Quick to cover 
sections and place in an 80 degree oven for 30 minutes or until 
hardened. 3. place slide in tissue floatation bath for 1 hour or until 
the section can be lifted off with forceps. 4. wet slide(s) that will 
receive the transferred section with water. 5 remove the section 
carefully. If the section does not come off easy, keep in waterbath 
longer. This is important--- the side that was on the old slide must be 
placed on the slide exactly how it was removed because this will have a 
flat surface. 6. dry in oven again for 1 hour 7. clear, dehydrate and 
use as needed. One problem I encountered was with plus slides. I had to 
leave the slide in the floatation bath overnight to get better results, 
otherwise, only parts of the tissue would transfer. JTT

Victor

Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
victor <@t> pathology.washington.edu
206-598-2792
206-598-7659 Fax
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Bull, Jennifer L. wrote:
> Does anyone have any experience in taking a stained H&E slide (non-charged), working  backwards to rehydration, and floating the individual tissue sections off into a waterbath to be picked up on a charged slide? I can't come up with any possible way to successfully do this, but one of our Pathologists says he has heard of it being done and wants us to do it. Any advice will be much appreciated.
>
>
> Jennifer Bull
> Histology Supervisor
> Northwest Pathology
> jennifer.bull <@t> nwpathology.com
>
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