[Histonet] CNS Fixation

[Breeden, Sara]

Yes, I'm back.  I need the expertise of this group.

 

I am trying to convince my three pathologists that fixation in alcoholic
formalin is the best route for whole brain and spinal cord.  This has
been an uphill battle and in order to prevent sections from peeling off
during staining, I am still pre-drying necropsy brain slides for an
extra 20 minutes before putting on the auto-stainer (even this does not
prevent all instances). I need some expert ammunition for my "battle"
despite the fact that the SOP I've written requires it and it is rarely
followed.  If alcoholic formalin is not as good as some other method,
I'm open to suggestion. Of course, convincing them that thinner sections
would also help - but one step at a time!  Thank you in advance!

 

Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 4700

Albuquerque, NM  87106

505-841-2576