[Histonet] mouse tibia 200um sections-low speed saw?

[Jamie E Erickson]

Hi Histonetters,
                               Can any one help.... I am trying to repeat 
what I see in this paper and it said it cut cortical mouse bone  tibia and 
I quote  "For the cortical bone, a pair of 100–200 um-thick
transverse sections were cut in the middle of the tibia with a low-speed 
diamond blade saw."

I tried to cut this thin but I can't not get that thin... I am using a 
buehler isomet low speed saw with a 4" 15HC  wafering blade. My micrometer 
reads every complete turn from zero to zero = 625um or 25 MIL and that is 
about as thin as I can cut mouse tibia. Please help am I missing 
something, what is the trick...??

Thanks

Jamie


_______________________________
Jamie Erickson
Sr. Research Associate II M.S. HTL (ASCP)
Department: DSMP
Abbott Bioresearch Center
100 Research Drive
Worcester, MA 01605-4341
508-688-3134
FAX: 508-793-4895
e-mail: jamie.erickson <@t> abbott.com