FW: [Histonet] decaling bones

[Ian Montgomery]

Hazel,
	Asked the boys here in Anatomy about your question and according to
them if you're using a propriety decal agent you have to be careful as the
bones do get harder if they are left in the solution. The calcium comes out
but the bones are really tough to cut. Interestingly, it's not such a
problem if using EDTA or acid, just the propriety agents.
Ian.     

Dr. Ian Montgomery,
Histotechnology,
I.B.L.S. Support Unit,
Thomson Building,
University of Glasgow,
Glasgow,
G12 8QQ.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Horn, Hazel
V
Sent: 14 April 2009 17:05
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] decaling bones

We are having the hardest time decaling bones.   Femurs and tibias.    

 

Is there such as thing as overdecaling and the bone becoming hard again?

 

Hazel Horn

Hazel Horn, HT/HTL (ASCP)

Supervisor of Histology

Arkansas Children's Hospital

1 Children's Way    Slot 820

Little Rock, AR   72202

 

phone   501.364.4240

fax        501.364.3155

 

visit us on the web at:    www.archildrens.org

 

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