[Histonet] TBS vs PBS

[Tony Henwood]

I am not sure of the effect of buffer type on the protease but it might
be possible that the proteinase K is the problem.
My experience is that they can vary between lots.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Charles,
Roger
Sent: Wednesday, 15 April 2009 12:41 AM
To: Histonet (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] TBS vs PBS 


Hello all,
Would proteinase K diluted in PBS be any different then PK diluted in
Tris buffer when used for a fluorescent antibody test?  I'm having
trouble duplicating a published testing method and the only difference
is PK diluted in TBS instead of PBS. Thanks in advance for all the
wonderful information from all. Roger

Roger Charles
Microbiologist
Pennsylvania Veterinary Laboratory
2305 N Cameron St
Harrisburg, PA 17110
717-787-8808
rcharles <@t> state.pa.us<mailto:rcharles <@t> state.pa.us>


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