[Histonet] processing artifact

[Rene J Buesa]

Gloria:
Frequently the problems that appear in the sections are attributed to processing defects when they usually originate before processing, during fixation.
Small pieces of tissue left to dry before being placed in the fixative. You write that sometimes happens to one biopsy in a group of several so consider the following: 4 biopsies are going to be taken and all are placed over gauze before being placed in the fixative. The one that was taken first was the one that had the greater chance to dry out totally or partially. The others stayed on the gauze less time, the one taken the first being the one that dried the most, the one that fixed less and the one perhaps that appears now with poor staining.
Check how those biopsy cases that you have problem now were taken, perhaps then you can find an explanation.
Hope this will help you
René J.

--- On Mon, 4/13/09, Okland, Gloria <gokland <@t> ameripath.com> wrote:

From: Okland, Gloria <gokland <@t> ameripath.com>
Subject: [Histonet] processing artifact
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Monday, April 13, 2009, 11:00 AM

We are seeing this opaque artifact in some tissues.  It can affect one biopsy
but not the two others in the same cassette.  It is very random, appears as this
white haze, obscuring nuclear detail, making the slides very difficult to read. 
Thinking it was water in the processor, I did a test run over the weekend and
everything looks great.  No reagents had been changed or adjustments made for
the test run.  Any suggestions of what else this could be and a remedy would be
greatly appreciated!!..

Thank you.

Gloria


Gloria Okland
AmeriPath | Histology

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