[Histonet]( FE on BM Cores)Effects of decalcification for special stains

[Akemi Allison-Tacha]

Don't forget the effects on decalcified Bone Marrow Cores.  Even if you use EDTA decalcification solution, there will be diminished FE staining on Bone Marrow Cores or any other tissue that is decalcified and then stained for FE.
Regards,Akemi

Akemi Allison-Tacha BS, HT(ASCP)HTL

Histology Manager

Associated Pathology Medical Group Laboratories

105A Cooper Ct. Los Gatos, CA 95032

Cell: 425.941.4287 

E-Mail: akemiat3377 <@t> yahoo.com

--- On Thu, 4/9/09, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:

From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Effects of decalcification for special stains
To: histonet <@t> lists.utsouthwestern.edu, "kyle ayres" <ayreskyle <@t> yahoo.com>
Date: Thursday, April 9, 2009, 1:53 PM

Usually not, unless the tissues remain too much time in the decalcifyier, meaning more time than needed to decalcify. For IHC the adverse effect is greater and because of that, when possible, the decalficication should be done with EDTA.
René J.

--- On Thu, 4/9/09, kyle ayres <ayreskyle <@t> yahoo.com> wrote:

From: kyle ayres <ayreskyle <@t> yahoo.com>
Subject: [Histonet] Effects of decalcification for special stains
To: histonet <@t> lists.utsouthwestern.edu
Date: Thursday, April 9, 2009, 3:31 PM

Are there negative effects associated with acid decalcification and special
stains, for example, AFB, GMS, and PAS (for fungus). All tissues are FFPE.
 
Thanks, 
Kyle Ayres HT BS
Nacogdoches Memorial Hospital  



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet