[Histonet] Effects of decalcification for special stains

[Rene J Buesa]

Usually not, unless the tissues remain too much time in the decalcifyier, meaning more time than needed to decalcify. For IHC the adverse effect is greater and because of that, when possible, the decalficication should be done with EDTA.
René J.

--- On Thu, 4/9/09, kyle ayres <ayreskyle <@t> yahoo.com> wrote:

From: kyle ayres <ayreskyle <@t> yahoo.com>
Subject: [Histonet] Effects of decalcification for special stains
To: histonet <@t> lists.utsouthwestern.edu
Date: Thursday, April 9, 2009, 3:31 PM

Are there negative effects associated with acid decalcification and special
stains, for example, AFB, GMS, and PAS (for fungus). All tissues are FFPE.
 
Thanks, 
Kyle Ayres HT BS
Nacogdoches Memorial Hospital  



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