[Histonet] Negative IHC controls

[Joe Nocito]

we're running one negative normal serum slide per block. For example, if 
we're performing mouse antibodies i.e. CD-45, HMB-45, pan Cytokeratin, we 
run one IgG mouse normal serum control. If we are running rabbit polyclonals 
(or rabbit monoclonals) and mouse monoclonals on the same case, we usually 
would run a IgG normal mouse and normal rabbit serum slide. Now, I know the 
purists out there are going to tell me what if I use an IgM mouse 
monoclonal, I should be running an IgM mouse normal serum. I agree, but my 
budget doesn't allow to run a negative for all scenarios. In that frame of 
mind, if we run a case with multiple different pretreatments, we run a 
negative on the harshest pretreatment. Again, not ideal, but I think it is 
better than just putting PBS or TBS buffer on a slide and calling that 
negative. That's my 3 cents worth and I'm sticking to that story.

JTT
----- Original Message ----- 
From: "Sheila Haas" <micropathlabs <@t> yahoo.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, April 08, 2009 1:57 PM
Subject: [Histonet] Negative IHC controls


I have a question concerning ANP.22570 on the CAP checklist. Could you all 
tell me how you are handling
negative controls for IHC staining? The question actually states (and I've 
confirmed with CAP) that we should be running two types of negative 
controls. One for reagents and one for each antibody in a run. I'd like to 
know
what the practice is. This seems very costly and time consuming. Thanks in 
advance!

Sheila Haas
Laboratory Supervisor
Micro Path Laboratories



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