[Histonet] MAMMOGLOBIN

[Jodie Robertson]

I use the Ultra View Detection kit on the Ventana Benchmark XT with the following protocol:

Deparaffinization
CC1 Mild
Mammaglobin - 32 min.
Ultra Wash
Hematoxylin - 4 min.
Bluing - 4 min.

We have very good results.  Mammaglobin isn't expressed in every breast case either, so the control could be the culprit.  We had to try a few tissues before we found one that yielded good results.


Jodie Robertson, HT (ASCP) QIHC
Pathology Sciences Medical Group
Histology Day Supervisor
183 E. 8th Ave.
Chico, CA  95926
530-891-6244 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Tuesday, April 07, 2009 10:36 AM
To: 'Vickroy, Jim'
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: AW: [Histonet] MAMMOGLOBIN

I tried also this antibody on Ventana Benchmark. Pity, I had no results.
Gudrun

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Vickroy,
Jim
Gesendet: Dienstag, 07. April 2009 17:54
An: 'Histonet <@t> lists.utsouthwestern.edu'
Betreff: [Histonet] MAMMOGLOBIN


Has anyone used the DAKO predilute antibody, Mammoglobin, on the Ventana
Benchmark XT?   And if so.....could you share the protocol you used?
Finally looking on the Dako website it appears that they have ready to use,
could you share with me which antibody should be used with the Ventana
I-view detection kit?

Thanks




Jim Vickroy BS, HT(ASCP)
Technical Supervisor - Surgical and Autopsy Pathology
Memorial Medical Center
217-788-4046
vickroy.jim <@t> mhsil.com



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