FW: [Histonet] Benchmark XT

[Esparza, Sandra]

Sandra

Sandra Esparza HT(ASCP)QIHC
Dell Children's Hospital of Central Texas
4900 Mueller Boulevard
Austin, Texas  78723
512-324-0000  x87061
sesparza <@t> seton.org


-----Original Message-----
From: Esparza, Sandra 
Sent: Wednesday, April 08, 2009 12:23 PM
To: 'gu.lang <@t> gmx.at'
Subject: RE: [Histonet] Benchmark XT

We had this happen to us.  It turned out that the reaction buffer and the EZ prep were mixed up.  We emptied all the carboids and started fresh.  The problem solved.  Hope this helps 

Sandra

Sandra Esparza HT(ASCP)QIHC
Dell Children's Hospital of Central Texas
4900 Mueller Boulevard
Austin, Texas  78723
512-324-0000  x87061
sesparza <@t> seton.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Wednesday, April 08, 2009 11:25 AM
To: 'Phyllis Thaxton'
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: AW: [Histonet] Benchmark XT

Phyllis,
we mainly don't have this problem and have been using the ultraview for at
least one year.
Do you airdry the slides horizontally? 
We only have the problem of loosing tissue, when the water under the
sections was not moved away well enough. And we often start the run directly
after cutting - no longer airdrying or oven.
I would recommend to put the slides directly after cutting in the 60°C oven
and dry vertically.

Gudrun


-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Phyllis
Thaxton
Gesendet: Mittwoch, 08. April 2009 15:44
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Benchmark XT

Is anyone out there having problems with tissue washing off the slides using
the Ultraview kit from Ventana? 

Mainly we are having problems with  needle biopsies washing off (prostate,
liver). Fixation and processing is always the same 4-6  hours fixation, 3
hour biopsy processing run on VIP processor, cut no thicker than 4 microns,
airdried at least 30 minutes, baked at 59-60 for at least one hour prior to
staining. The instrument's calibrations have been checked and are fine. 

We never had this problem with the iView kit using the Nexes, doing
pretreatments offline.

Any help, ideas, info will be appreciated.

Thanks,
Phyllis Thaxton HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL 35401


      
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