[Histonet] silanising slides

Patsy Ruegg pruegg <@t> ihctech.net
Sun Apr 5 12:57:30 CDT 2009


Is your solution supposed to be diluted or used neat?  I agree with Rene,
the solution will coat the slide and can be used until it is used until it
is gone, it does not go bad.
Patsy


Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
email pruegg <@t> ihctech.net
website www.ihctech.net
IHC Resource Group www.ihcrg.org 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Sunday, April 05, 2009 11:52 AM
To: triple immunohistochem; anjan kumar
Subject: Re: [Histonet] silanising slides

Until your solution is used up. The solution is homogeneous and immersing a
slide inside it will just take the corresponding amount of silane. Different
thing with staining solutions that are usually weak and last for a limited
number of slides.
René J.

--- On Sun, 4/5/09, anjan kumar <drvet_anjan <@t> hotmail.com> wrote:

From: anjan kumar <drvet_anjan <@t> hotmail.com>
Subject: [Histonet] silanising slides
To: "triple immunohistochem" <histonet <@t> lists.utsouthwestern.edu>
Date: Sunday, April 5, 2009, 12:41 PM

hi everyone,
                 can any one tell me how many slides can be processed with
10ml
of  3-aminopropyltriethoxysilane. i have a 100ml solution from Sigma.

Regards,
Dr. Anjan Kumar.K.R

M.V.Sc Scholar

Dept. of Veterinary Pathology

Madras Veterinary College

Chennai-7

India

email: drvet_anjan <@t> hotmail.com

Phone: +91-9940475801



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