[Histonet] Entamoeba Trophozoites

[Rene J Buesa]

Lynette:
Since it was received in saline and the tissue has its normal consistency I would prepare a squash (squash the tissue between 2 glass slides not between coverslips) with a rotary movement so you have 2 large areas of squashed tissue. After that, air dry, fix with methanol, air dry again and stain with Giemsa.
That is what I would do. Try it if you do not have a better option.
René J.

--- On Fri, 4/3/09, Lynette Pavelich <lpaveli1 <@t> hurleymc.com> wrote:

From: Lynette Pavelich <lpaveli1 <@t> hurleymc.com>
Subject: [Histonet] Entamoeba Trophozoites
To: histonet <@t> lists.utsouthwestern.edu, histonet-bounces <@t> lists.utsouthwestern.edu
Date: Friday, April 3, 2009, 3:09 PM

I need help!  Just received a needle biopsy of liver submitted in normal
saline.  They did all the routine cultures/ fungal, and are requesting a
wet mount on this tissue for Entamoeba Trophozoites.  
I have contacted all of my resources in and out of state, and they
cannot help me. 

HELP!!

thanks, 
Lynette

Lynette Pavelich, HT(ASCP)
Histology Supervisor
Hurley Medical Center
One Hurley Plaza
Flint, MI  48503

ph:  810-257-9948
fax:  810-762-7082

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