[Histonet] Embedding cell pellets

[Bernice Frederick]

Try histogel from Richard Allan- available from Fisher.Easier than making up
agarose!
Bernice


Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL 
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Thomas Pier
Sent: Thursday, April 02, 2009 11:18 AM
To: histonet <@t> lists.utsouthwestern.edu; Louise_Hartson <@t> URMC.Rochester.edu
Subject: Re: [Histonet] Embedding cell pellets

Fix the cells, spin them down, decant, and resuspend in 1% agarose.  The
agar plug can be processed and embedded like any other piece of tissue.

Tom Pier

>>> "Hartson, Louise" <Louise_Hartson <@t> URMC.Rochester.edu> 04/02/09 11:08 AM
>>>

I am looking for a protocol for embedding cell pellets in paraffin.
Thanks,
Louise

Louise Hartson, BA
Senior Technical Associate
University of Rochester
Louise_Hartson <@t> URMC.Rochester.edu
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